Abstract: In order to elucidate the roles of DHRS7 gene in formation of goose fatty liver, the goose DHRS7 gene full-length cDNA sequence was cloned by RACE method, and then was analyzed by bioinformatics method. Meanwhile, expression of DHRS7 in goose different tissues and organs was analyzed using qRT-PCR method, as well as the mRNA expression influenced by forcing-feed was analyzed in goose liver. Results showed that the full sequence of goose DHRS7 gene was 1 297 bp, including an coding domain sequence (CDS) encoding for 336 AA, and a 25 bp 5′ UTR and a 243 bp 3′UTR. Results of bioinformatics analysis showed that the geese DHRS7 posses a transmembrane region and a NADP binding region, on which functional region there is few variants. The DHRS7 had a higher expression level in geese liver and adipose tissues. And the expression of DHRS7 up-regulated significantly in geese liver tissues after a force feeding (P<0.05). The results indicate that the goose DHRS7 gene may play an important role during formation of geese fatty liver.