ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2012, Vol. 43 ›› Issue (9): 1369-1376.doi:

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Developmental Expression of PPARα and PPARγ mRNA in Adipose Tissues of Different Fat-tailed Sheep

LIN Pei-pei, GAO Zhong-yuan, YUAN Ya-nan, WU Jian-liang, LIU Bao-feng, ZHOU Sha-sha, QIAO Li-ying, LIU Jian-hua, LIANG Chen, LIU Wen-zhong*   

  1. (College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China)
  • Received:2011-12-23 Online:2012-09-25 Published:2012-09-25

Abstract: The objectives of this study were to investigate the developmental expression characteristics of PPARα and PPARγ mRNA in two sheep breeds of distinct tail types and to discuss the probable relationship between the mRNA expression and metabolism of adipose tissue. Guangling Large Tailed (GLT) sheep and Small Tailed Han sheep (STH) at 2, 4, 6, 8, 10 and 12 month old were used to detect the mRNA expression in seven adipose tissues (great omental, small omental, tail, subcutaneous, mesenteric, perirenal and retroperitoneal fat) by real-time PCR. The results showed that PPARα and PPARγ expressed in all tissues studied. Generally, the mRNA expressed lower in superficial fats than that in deep fat deposits. As the main effects, months of age and breed did not basically influence the mRNA expression of PPARα and PPARγ. Though sex did not influence PPARα expression in both breeds, low expression of PPARγ in females of GLT resulted in the significant sex effect and interaction between sex and breed. Based on the expression differences between tissues and ages, respectively, the expression of the two genes was of significant spatiality but not of temporality. The two genes had a synergistic effect on the metabolism of adipose tissues despite their opposite functions.

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