Abstract: The purpose of this study was to investigate the hereditary basis of high prolificacy in goat, the DDRTPCR was applied to screen 4 gene fragments differentially expressed as candidate genes affecting the reproductive traits in the ovarian tissues of local native goat of the midst of Hebei with different fecundity levels during oestrum. According to the further analysis result of homology and transcription factor binding sites, two segments were newly found. One had 81% homology with cloned fragment (THY010003E05) of wild boar, but the function was unknown. Another segment was shared 94% homology with the pcnp mRNA gene in cattle. Accordingly, the pcnp gene of goat was amplified, cloned and sequenced. By using bioinformatics softwares and methods, the chemicophysical properities, secondary structure, signal peptide, nucear localization sequence, phosphorylation sites, transmembrane region and protein functional domains of the amino acid sequences coded by pcnp gene were predicted. Compaerd with complete CDS sequence of pcnp gene of cattle,the sequence homologous of pcnp in goat was 100%, coding region was 537 bp encoding 179 amino acids. It was suggested that PCNP was a hydrophilic nucleoprotein.Random coils and αhelix were the main structural type of the flexible region in secondary structure, and there were partial extended chains and βturn. 5 proteins interacting with PCNP protein were predicted, and 3 low complexity regions were discovered. It was suggested that PCNP was relatively conservative, it was most likely to play a role in the nucleus and affect goat prolificacy via involving in cell cycle regulation or gene expression.