ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2012, Vol. 43 ›› Issue (3): 453-459.doi:

• 预防兽医 • Previous Articles     Next Articles

Cloning and Sequence Analysis of the ITS and 5.8 S rDNA of Larval Eustrongylides spp. Isolates from Monopterus albus (zuiew)

DUAN Liuchun1，LIU Wei1，XU Pingyuan2，HE Xin1，ZHOU Xiaojun1，
LIU Zhilian1，ZHENG Yanqiong1，LIU Yi1*   

  1. 1.College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128，China；2. Hunan Biological Electromechanical Vocational Technical College, Changsha 410128，China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-03-28 Published:2012-03-28
  • Contact: LIU Yi

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Abstract: The objectives of the present study were to examine sequence variation in the internal transcribed spacer (ITS) and 5.8S rDNA of Eustrongylides spp. isolates from Monopterus albus, and to reconstruct their phylogenetic relationship using ITS sequences. The ITS sequences were amplified from each Eustrongylides spp. samples and the amplicons were cloned into pGEMT Easy vector, respectively. The inserts were successfully sequenced, and the length of ITS in 32 Eustrongylides spp. isolates were different（922927 bp）. Sequence analysis revealed that the ITS1, 5.8S and ITS2 rDNA of these samples were 350351 bp, 102 bp and 340344 bp in length, respectively. The results indicate that the ITS sequences provide useful genetic marker for molecular identification of Eustrongylides spp. It was the first time that the complete sequence of ITS and 5.8 S rDNA of Eustrongylides spp. were reported. The results of this study lay down the foundation for further study on molecular epidemiology and diagnostics of Eustrongylides spp.