Abstract: The aim of this study was to separate subsets proteins in uncapacitation and capacitation of boar sperm cells and to identify the protein tyrosine phosphorylation, and to establish the theoretical foundation for the mammalian sperm fertilization biology research．In vitro，the boar sperms were cultivated for capacitation and separated subsets proteins．The tyrosine phosphorylated proteins in uncapacitation and capacitation sperms were identified by using SDSPAGE and Western blot．In capacitation boar sperm, the degree of tyrosine phosphorylation on 126, 108, 79 ku proteins were significantly higher than them on uncapacition boar sperm. The vigor indexes and the results of SDSPAGE of uncapacitation and capacitation sperms were different，all the vigor indexes of capacitation sperms were significantly improved and these changes were related with tyrosine phosphorylation. The molecular weight of tyrosine phosphorylated proteins on membrane were approximately 25, 47 and 50 ku and on cytoplasm is about 47 ku． The degree of tyrosine phosphoproteins of 25 and 47 ku in capacitation sperm were significantly higher than them in the uncapacitation sperm（P<0.05）. This phenomenon also existed in cell nucleus．In sperm nucleus，these proteins’ molecular weight were 23, 37 and 4250 ku．The degree of tyrosine phosphrylated 23 ku protein in nucleus of capacitation sperms are higher．The results suggests that the main tyrosine phosphorylated proteins were on the nucleus and membrane proteins，and a few of them are plasma proteins.