Abstract: In this study, it was aimed to investigate the distribution of luteinizing hormone receptor (LHR) on the ovaries among three different sheep breeds by different efficiency of superovulation. Three local sheep breeds (Smalltailed Han sheep, White Tan sheep and Ujumqin sheep) were selected depending on their different sensitivity to superovulation by LH as following: Smalltailed Han sheep, White Tan sheep, and Ujumqin sheep. At 8, 16, 24 hour after the last injection with LH, the sheep ovaries were collected and treated by immunohistochemical staining to observe the distribution of LHR on their different ovarian follicles. The results showed that: ① LHR were expressed in both granulosa and theca cells of antral follicles，but did not express on the preantral ones. ②At 8, 16, 24 hour after the last injection with LH, the LHR levels on the prematured follicles of the three local sheep breeds were significantly higher than those on the corresponding secondary ones respectively（P<0.05）. ③The expression change of LHR in the prematured follicles had significant difference among the three sheep breeds, the order as following: Smalltailed Han sheep＞White Tan sheep＞Ujumqin sheep（P<0.05）. The result indicate that: ①The induction of LH for the development of the primary ovarian follicles may not be through the specific LH receptor signal way, but through some other nonspecific ones. ②The expression change of LHR in the prematured follicles is consitent with the effect of superovulation, which indicate that the effect of superovulation is linked with the distribution of LH receptor on ovaryes in sheep.