ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2013, Vol. 44 ›› Issue (7): 1000-1007.doi: 10.11843/j.issn.0366-6964.2013.07.001

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The Partial cSNPs Detection of Pig BPI Gene and Its Influence on Protein Structure and Function

WU Zheng-chang1, WANG Jing1, ZHU Shi-ping1, ZHAO Qiao-hui1, LIU Lu1, ZI Chen1,WU Sheng-long1,2*, BAO Wen-bin1,2*   

  1. (1. Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of JiangsuProvince, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009,China; 2. Jiangsu Engineering Research Centre for Molecular Breeding of Breeder Pig, Changzhou 213149, China)
  • Received:2012-12-24 Online:2013-07-23 Published:2013-07-23

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Abstract:

This study was conducted to detect the single nucleotide polymorphism (SNPs) of BPI gene in partial coding region in ninety-eight Sutai pigs, and to predict the effect of mutation on BPI protein structure and function.PCR-SSCP/RFLP combined with sequencing were used to detect the SNPs in exon 1, exon 4 and exon 10 of BPI gene, and bioinformatics approach was used to predict the physicochemical properties and structures of original protein or mutant protein. Refer to the cDNA sequence of BPI gene, six SNPs existed in exon 1, exon 4 and exon 10 of BPI gene, with three synonymous mutations（c.24A>G , c.54T>C and c.522C＞T） and three missense mutations (c.433C>T(Arg145Trp), c.1060A>G(Thr354Ala) and c.1151T>G(Leu384Arg)). Three missense mutations had no influence on the physicochemical properties, signal peptide, transmembrane region, glycosylation sites, secondary structure and tertiary structure of the BPI protein，but led to the change of its phosphorylation sites or the existence of other potential function sites domain. The result of Western blot experiment indicated that there was an target band at 53 ku. It was obvious that expression of BPI protein in ETEC F18-resistant group was significant higher than that in susceptible group. The base mutations in exon 4 and exon 10 of pig BPI gene affect protein function in one way or another, which may have an influence on resistance/susceptibility of the pig disease.

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