ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2010, Vol. 41 ›› Issue (6): 705-710.doi:

• 预防兽医 • Previous Articles     Next Articles

Gene Cloning and Bioinformatics Analysis of Outer Capsid Protein VP7 genefor Giant Panda Rotavirus CH-1 Strain

YAN Qi-gui1,4*, LEI Yan1, ZHANG Zhi-he2, WANG Cheng-dong2,
YANG Ai-guo3, WANG Xu1, ZUO Lan1, XIAO Yang5   

  1. 1. Animal Biotechnology Center of Sichuan Agricultural University, Ya′an 625014, China;2. Chengdu Research Base of Giant Panda Breeding, Chengdu 610081, China; 3. Provincial Center of Epizootic Prevention & Control of Sichuan, Chengdu 610041, China; 4. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya′an 625014,China; 5. College of Life and Science, Sichuan Agricultural University, Ya′an 625014, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-06-25 Published:2010-06-25

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Abstract: The VP7 gene coding outer capsid protein of giant panda rotavirus（GPRV）strain CH-1 was cloned by RT-PCR from GPRV infected MA-104 cells，and sequencing and bioinformatic analysis were then conducted to investigate the structure and function of GPRV VP7 protein. The function of this protein was predicted by bioinformatics software and the phylogenetic tree of VP7 gene was constructed by CLUSTALW software. The results indicated that a length of 1 042 bp GPRV VP7 protein encoding gene was obtained（GenBank accession number：GU188284）. Bioinformatics analysis showed that the sequence contains the complete coding sequence of VP7 protein, which is 981 bp, encoding 326 aa. The molecular weight, isoelectric point, estimated halflife, instability index of GPRV VP7 were 37 354.8 Da, 4.76, about 30 hours and 26.71, respectively. Grand average of hydropathicity of VP7 protein was -0.015, while the hydrophobicity was between -2.344 and 3.567. The protein has one signal peptide between 1 and 24 site, two transmembrane regions 4—23 site and 33—53 site and 12 antigenic determinants predicted by online analysis. Motif searching showed that VP7 protein may have 2 Nglycosylation sites, 5 protein kinase C phosphorylation sites, 4 casein kinase Ⅱ phosphorylation sites, 1 tyrosine kinase phosphorylation site, 4 Nmyristoylation sites, 1 prokaryotic membrane lipoprotein lipid attachment site and 1 grampositive cocci surface proteins “anchoring” hexapeptide. The phylogenetic tree showed that the evolution distance of giant panda rotavirus VP7 gene is homogeneous to human rotavirus A. The research obtained the clone of GPRV VP7 gene, and displayed some new clues for further studying the biological functions of the gene and establishing diagnosis method of the virus.