ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2011, Vol. 42 ›› Issue (9): 1207-1212.doi:

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Cloning, Expression and Cold-stimulation Expression of Y-box Binding Protein Gene in Min Pig

ZHANG Dongjie1, LIU Di1,2*, WANG Liang1, ZAHNG Xu2, BIE Shu2, SUN Hongtao2, YANG Guowei1

  

  1. 1.Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China; 2. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-09-23 Published:2011-09-23
  • Contact: LIU Di

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Abstract: This experiment was conducted to study whether Ybox binding protein participate the coldadaptive response of mammalian. The RTPCR and Realtime PCR methods were used to clone and sequence the YB1 gene of Min pig. The expression level of YB1 in large intestine, leg muscle, lung, fat, heart, spleen and liver was detected. The expression change between ordinary temperature group ((10±2)℃)and low temperature group ((-20±3)℃) after thirteen days coldstimulation in skeletal muscle was compared. The results showed that the complete CDS of Min pig YB1 was 975 bp, encoding 325 amino acids, having a highly conserved cold shock domain, existing some phosphorylation sites, which didn’t contain any transmembrane domain, and was a protein found in the cytoplasm. Tissue expression pattern showed that the YB1 gene was highly expressed in tissues detected. The expression level of YB1 in leg muscle was decreased significantly after thirteen days coldstimulation (P＜0.05). The biology role of YB1 during coldadaptation could not be determined, but the coldstimulation really caused changes in the YB1 gene transcription.