ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2010, Vol. 41 ›› Issue (4): 410-416.doi:

• 遗传繁育 • Previous Articles     Next Articles

Effect of TSA on Fibroblast Cell as Donor Cell in the Tan Sheep

LI Xiangchen 1, YAO Yaxin 1,2, LIANG Suli 1,3, WANG Yipeng 1,4,
GUAN Weijun 1*, MA Yuehui 1   

  1. 1. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;2.College of Wildlife Resource, Northeast Forestry University, Harbin 150040, China; 3. College of Animal Science and Technology, Northwest A﹠F University, Yangling 712100, China; 4. College of Animal Science and Animal Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China
  • Received:2009-05-11 Revised:1900-01-01 Online:2010-04-25 Published:2010-04-25
  • Contact: GUAN Weijun,MA Yuehui

PDF (PC)

471

Abstract: In order to investigate the potential of fibroblasts as donor cells, and find out the optimal concentration of TSA used to treat fibroblasts, and further to improve the developmental level of cloned embryos, the fibroblasts were treated with 100, 50, 25, 10 ng·mL-1 TSA, cell morphology and viability were investigated. Flow cytometry were adopted to analyse cell cycle. Acetylation level was detected by indirect immunofluorescence, and the developmental level of cloned embryos was evaluated. Most cells treated with 100 ng·mL-1 TSA were dead, but a significant increase of acetylation level was detected; the cell cycle was arrested at G0/G1 checkpoint with the existence of TSA; generally，there was a higher proportion of cleavages and blastulas (P<0.05, (85.2±3.4)% vs( 68.6±6.7)%, (35.6±5.7)% vs (10.4±8.3)%) when the cells were treated with 10 ng·mL-1 TSA. The results suggested that acetylation level, cell morphology and embryonic developmental level were improved and optimal for the donor cells treated with 10 ng·mL-1 TSA.