Abstract: An unknown gene, SLC10, was cloned by RT-PCR from Cysticercus cellulosae on the base of transspliced leader sequence. The SLC10 gene was cloned from vector of pGEM-SLC10 and inserted into pPIC9k to construct a recombinant secretory vector named pPIC9K-SLC10. Then the pPIC9k-SLC10 was transformed into E.coli JM109.The recombinant vector was identified by PCR, enzymatic analysis and sequencing. The positive plasmid was linearized with Sac I and transformed into GS115 by electroporation and the insert was integrated into genomic DNA of Pichia pastoris by means of homologous recombination. The recombinants with multiple integrated copies of SLC10 were screened using gradient concentration G418 and induced with methanol. The results of SDS-PAGE and Western-blot showed that the expressed protein was not of immunoreaction.