ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2008, Vol. 39 ›› Issue (7): 994-999.doi:

• 研究简报 • Previous Articles     Next Articles

DNA Segment Cloning and Bioinformatics Analysis of SREBP-1 Gene in the Landes Goose

HAN Chun-chun, WANG Ji-wen*   

  1. Key Lab of Animal Genetic Resources, Sichuan Agricultural University, Ya’an 625014,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-07-23 Published:2008-07-23

Abstract: Total RNA from Landes goose liver of 16 weeks old was extracted.The cDNA segment about 282 bp of Langdes goose was amplified by the reverse transcription-polymerase chain reaction(RT-PCR)Sequence analysis showed that there is a high similarity between the fragment of cDNA and sterol regulatory element binding protein-1(SREBP-1) gene containing a bHLH function region. Meanwhile, the segment evidently has a hydrophilic region and a hydrophobic region, and the secondary structure richly contains αhelix, which accords with its function.