ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2005, Vol. 36 ›› Issue (6): 545-549.doi:

• 畜牧 • Previous Articles     Next Articles

Analysis of Genetic Polymorphisms at Five Loci in Xi’an Population of Chinese Holstein Cattle by PCR-RFLP

ZHANG Run-feng;CHEN Hong;LAN Xian-yong;TIAN Yi;PAN Chuan-ying;HU Shen-rong;SU Li-hong   

  1. 1.College of Animal Science and Technology, Northwest Science and Technology University of Agriculture and Forestry, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling 712100, China; 2.Institute of Biotechnology, Xuzhou Normal University, Xuzhou 221116, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2005-06-25 Published:2005-06-25

PDF (PC)

439

Abstract: The genetic polymorphisms of Xi’an population of Chinese Holstein cattle were detected for κ-cn, β-lg, β-lg 5′flanking region, CSN1S2F and IGFBP-3 genes by PCR-RFLP. No carriers of the allele of CSN1S2F were found and its polymorphism information content was 0. κ-cn locus showed low polymorphism (PIC=0.236 6), the three loci of β-lg, β-lg 5′flanking region and IGFBP-3 genes moderate polymorphisms (0.316 8, 0.368 9 and 0.443 9 respectively). The heterozygosity (h) and DNA polymorphisms of the five loci (κ-cn,β-lg,β-lg 5′flanking region, IGFBP-3 and CSN1S2F genes ) were 0.274 2, 0.394 7, 0.487 9, 0.489 1, 0.000 0 and 0.025 5, 0.011 6, 0.033 3, 0.011 2, 0.000 0 respectively. And κ-cn, β-lg, β-lg 5′flanking region and IGFBP-3 loci in the population were at Hardy-Weinberg equilibrium. Only BB genotype was found at CSN1S2F loci.