Abstract: To investigate the effects of melatonin（MT）on the function of liver mitochondria in goats with endotoxemia，48 normal goats were divided randomly into four groups：saline（SL）group,LPS group,MT group and MT+LPS groupFour groups were treated with saline（SL）, LPS（1 mg·kg^-1）, MT（1 mg·kg^-1）and MT+LPS，respectively.After injection of drugs，livers were collected from 6 killed goats per group at the 3rd hour and 6th hour，respectively.Liver mitochondria and mitochondrial DNA （mtDNA） were extracted for evaluation of mitochondrial membrane potential（MMP）,activity of adenosine triphosphatase（ATPase）,content of Cardiolipin（CL）and level of 8-hydroxy-2′-deoxyguanine（8-OH-dG）in mtDNA.Results showed that：compared with SL group， MMP of LPS group was obviously declined，activity of ATPase was restrained， content of CL decreased greatly，level of 8-OH-dG increased significantly；but compared with LPS group，MMP of MT+LPS group was raised obviously， activity of ATPase was significantly recovered， content of CL increased greatly，level of 8-OH-dG decreased apparently.The experimental results indicated that melatonin could reduce liver mitochondrial damage induced by endotoxin， and could protect liver mitochondrial function in goats with endotoxemia.