Abstract: RFLP technique was applied in this study to analyze the distribution of MyoD gene in 10 different pig breeds and pig breed crosses. The population genetic information about genetic distribution, variation, and heterozygosity of MyoD gene in different breed population was analyzed. Based on allele frequency, genetic distance and evolution distance among each breed populations were calculated and UPGMA phylogenetic tree was got based on the evolution distances between populations. The results indicated that the distribution of MyoD genotype keep in HardyWeinberg equilibrium in most testing groups but not in Duroc and DLY population. Generally speaking, the genetic diversity of MyoD gene was abundant and these testing breed groups with high genetic variation. The evolution of MyoD gene was under natural selection pressure. The 10 pig breeds were divided into 4 clusters. The first cluster was consisted of four breeds developed from Landrance, the second cluster was two Chinese indigenous pig breeds, the third cluster was three breeds developed from Duroc and the fourth cluster was Tibetan pig breed. The phylogenetic tree was consistent with breeding facts of each breed. From this experiment, we concluded that some RFLP data from functional gene could be used in the evolution research of some closely related species.