Abstract: The regularity of IgA, IgG and IgM antibodies in local mucosal and systemic immunity of ducks induced by attenuated duck plague virus vaccine strain vaccinated orally were studied in this paper. Twenty-day-old Cherry Valley ducklings were vaccinated orally with attenuated duck plague virus Cha strain and every five vaccinated ducklings were killed punctually in 60 days, and DPVspecific antibodies were detected in serum, bile and the secretion from trachea and enteron (oesophagus, duodenum, jejunum, ileum, caecum and rectum) by indirect ELISA(results were presents as the log10 geometric mean antibody titer±SD).The results showed that the order of antibody titers from high to low was:① IgG, IgM and IgA in serum, and could be detected at 9-60, 3-15 and 9-36 days respectively;②IgA, IgG and IgM in bile,and could be detected at 6-15, 12-36 and 9-12 days respectively;③IgA, IgM and IgG in trachea, and could be detected at 6-60, 3-12 and 9-27 days respectively;④IgA, IgM and IgG in oesophagus and could be detected at 3-60, 3-27 and 9-60 days respectively; ⑤The antibody titers in intestines from high to low and the period of time when the antibodys could be detected were respectively as follows: IgA(3-60 days),IgM(3-15 days)and IgG(9-27 days) in duodenum; IgA(6-60 days),IgM(6-12 days)and IgG(9-36 days) in jejunum; IgA(9-60 days),IgM(6-9 days)and IgG(15-21 days) in ileum; IgA(6-60 days),IgG(12-36 days)and IgM(6 days) in caecum; IgA(3-60 days),IgG(12-21 days) and IgM(6 days) in rectum. Data presented here indicated that IgM was produced firstly and IgG was the most important antibody in systemic immunity, and IgA, IgG and IgM preserved in the bile to be short, and IgA was the most important antibody in trachea and enteron after the duckling were vaccinated orally with attenuated duck plague virus strain.