Abstract: Using genome scanning method, three missense mutation sites of Lpin1 gene located in the coding region were found, which were c.391G>A mutation(Asp131Asn), c.1727C>T mutation(Ala576Val) and c.2287A>C mutation(Met763Leu). Three pairs of primers were designed according to amplification-created restriction site PCR(ACRS-PCR)methods and were used to amplify the fragments covering the corresponding mutation. The PCR products were distinguished by the restriction fragment length polymorphism(RFLP)method. The association analysis between different genotypes and fattiness traits were performed in Tibetan chicken, Recessive White, crossbreeds of Tibetan♂×Recessive♀and Tibetan♂×(Tibetan♂×Recessive♀)populations. The genetic diversity about mutation sites was analyzed in 5 native chicken breeds(Gushi, Xiaoshan, Beijing Fatty, Silky Sooty and Chahua). The results showed that the polymorphism of the Lpin1 c.1727C>T mutation was apparently abundant in different chicken breeds while the others were monotonous. It revealed that the frequency of C allele in c.1727C>T mutation site was higher than that of A allele, and the distribution pattern of c.1727C>T locus genotype in the 5 chicken populations obeyed the Hardy-Weinberg equilibrium through the χ2 test, except the Gushi chicken and the Beijing Fatty chicken. The association analysis showed that there were a significant association between the c.1727C>T site polymorphism and subcutaneous fat thickness(P<0.05), and abdominal fat weight(P<0.01), and also revealed that the chickens with CC genotype had a higher subcutaneous fat thickness than chickens with the CT or TT genotype. Meanwhile, the chickens with TT genotype had a higher abdominal fat weight than chickens with other genotypes(CT or TT).