ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2006, Vol. 37 ›› Issue (10): 1078-1083.doi:

• 研究简报 • Previous Articles     Next Articles

Isolation and Identification of a Nonhaemagglutinating Strain of RHDV from China and Cloning and Sequence Analysis of VP60 Gene of the Strain

TIAN Lang;WANG Hong-ning;LI Jian-wen;LIAO Juan;ZHANG Xia-lan;ZHOU Wan-rong;WANG Hong-bin

  

  1. 1.ollege of Animal Science and Technology，Sichuan Agricultural University，Yaan 625014，China；2.ollege of Life Scince, Sichuan University, Chengdu 610064, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-10-25 Published:2006-10-25

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Abstract: A variant strain of rabbit hemorrhagic disease virus which was named “Yaan-1” was isolated and identified in China The virus lacked HA activity at 25,37 and 4 ℃,but gave positive results in agar diffusion reaction(ADR) and counter immunoelectrophoresis (CIE) The capsid protein（VP60）of Yaan1 strain was cloned by RT-PCR and sequenced VP60 gene of Yaan-1 was 1 740 nt in size and encoding 579 aa Alignment with other 14 sequences of RHDV isolates in the world showed that the homology were 90.0%-98.0% for nucleotide sequence，and 94.1%-99.0%for amino acid sequence respectively The results indicated that the sequence of VP60 gene of different RHDV isolates, including nonhaemagglutinating Yaan-1 strain, were highly homologous The hydrophilicity plot, flexible regions, antigenic index of VP60 protein of Yaan-1 strain, etc were also analyzed