Abstract: Microsatellite enrichment from AFLP fragments by magnetic beads can obviate the tedious work of library construction which save not only time but also expense. The genomic DNA was converted into pre-amplified AFLP fragments by using a few restriction enzymes combination and hybridized with biotinlabeled SSR probes. Then the hybrid mixture was used to incubated with magnetic beads coated with streptavidin. After washing to remove the non-SSR fragments, the eluted single-strand DNA which was cloned and sequenced was largely enriched for microsatellites. Primers can then be designed according to the sequence flanking the repeat motifs and used for polymorphism analysis. Thirty primers had been developed from six China indigenous geese breeds successfully. Eleven of them were highly polymorphic better specific and did not link each other. The mean Polymorplism Information Content(PIC) of six geese breeds was also all over 0.5. The highest was the Gushi goose (0.546), and the lowest was the Yan goose (0.513). The results showed that the 11 microsatellites had higher PIC . These 11 microsatellites could be used in the analysis of genetic diversity, construction of saturated maps, and in some cases, in marker-assited selection.