ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2005, Vol. 36 ›› Issue (10): 1038-1042.doi:

• 兽医 • Previous Articles     Next Articles

Study on Comparison of Expressing Characteristics of Classical Swine FeverVirus and Fulllength cDNA in Pocine Kidney Cells

TIAN Hong; ZHANG Yan-ming;LIN Tong; LIU Xiang-tao;HU Jian-he;WU Jing-yan;ZHANG Miao-tao;XIE Qing-ge   

  1. 1.Northwest Sci-tech University of Agriculture and Forestry，Yangling 712100，China;2. Lanzhou Veterinary Research Institute，Chinese Academy ofAgricultural Sciences, Lanzhou 730046, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2005-10-25 Published:2005-10-25

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Abstract: Some characteristics of multiplication and expression of classical swine fever virus Cstrain,Shimen strain and constructed viral RNA were studied by RT-PCR, direct fluorescent antibody method, sandwich ELISA. The results indicated that Shimen strain can easily adapted SK6 cell;but C strain and constructed viral RNA were sensitive to PK15 cell.Meantime RT-PCR, direct fluorescent antibody method, sandwich ELISA is the useful methods for identifying the transfected cell.