ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2005, Vol. 36 ›› Issue (10): 1028-1032.doi:

• 兽医 • Previous Articles     Next Articles

Development and Optimization of an ErnsELISA Based on RecombinantErns Protein for Detection of Antibodies against Classical Swine Fever

JIA Hong-lin; QIU Hua-ji; ZHU Qing-hu;LI Guo-xin;LI Na;LUO Yu-zi;LIU Yong-gang; LI Yan;TONG Guang-zhi   

  1. 1. National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences, Harbin 150001,China; 2. Agricultural College of Yanbian University, Longjing 133400, China; 3. College of Veterinary Sciences, Xinjiang Agricultural University, Urumqi 830052, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2005-10-25 Published:2005-10-25

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Abstract: This study aims to develop a differential assay for popularizing the use of E2-based marker vaccines against classical swine fever (CSF). Based on the recombinant Erns produced by E. coli, an indirect ELISA (Erns-ELISA) was established and optimized using the Erns fusion protein as coating antigens. The optimal amount of coating antigen was optimized to be 0.15 μg, and the serum dilution was optimized to be 1∶100. The established Erns-ELISA was shown to be sensitive, specific and reproducible, and has a good agreement with E2-ELISA based on the recombinant fusion E2 protein (96.7%) and Dot-ELISA based on whole viral antigens (77.5%). The Erns-ELISA combined with E2-ELISA can be used as differential diagnostic assays accompanying marker or DIVA vaccines against CSF to differentiate animals infected with wild-type viruses from those vaccinated with E2-based marker vaccines.