ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2009, Vol. 40 ›› Issue (2): 221-227.doi:

• 预防兽医 • Previous Articles     Next Articles

Cloning and Sequence Analysis of the HA，NP，NA，M and NS Genes of H1N2 Swine Influenza Viruses

MENG Xue-qiong1，CHEN Yi-xiang2*，LIU Qi2，ZHENG Min2，SHI Kai-chuang2, HU Jie2   

  1. 1. College of Animal Science and Technology， Guangxi University， Nanning 530005，China;2. Guangxi Center for Animal Disease Control and Prevention， Nanning 530001， China
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-02-24 Published:2009-02-24

PDF (PC)

666

Abstract: In this study，the HA， NP， NA， M and NS genes of three isolates of H1N2 swine influenza viruses (SIV) were cloned and sequenced. Sequence comparisons revealed that three isolates share a higher degree of similarity. Genetic analysis demonstrated that three isolates were closely related to the United States triple reassortant H1N2 SIV isolate. In the HA， NP， M and NS genes phylogenetic trees， three isolates clustered with classical H1N1 SIV. While in the NA gene tree， isolates clustered with human influenza viruses. Comparisons of deduced amino acid sequences of HA and NA of the 3 isolates with the representative viruses A/swine/Maryland/23239/1991 (H1N1) and A/Buenos Aires/4459/96 (H3N2) revealed that they shared 954%961% (HA) and 966%972% (NA) amino acid similarities， respectively. Amino acid substitutions were detected at previously defined glycosylation sites， antigenic sites and receptor binding sites of the HA or NA proteins. The effects of isolates owing to these amino acid substitutions on biological characterization are unknown.