ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2007, Vol. 38 ›› Issue (1): 53-58.doi:

• 预防兽医 • Previous Articles     Next Articles

Construction of Recombinant Virus Strain TK-/gG-/PrM+ Recombinized by Pseudorabies Virus Ea Mutant Strain TK-/gG-/LacZ+ and PrM Gene of Japanese Encephalitis Virus

  

  1. 1College of Veterinary Medicine， Huazhong Agricultural University,Hubei Province’s Key Laboratory of Preventive Veterinary, Wuhan 430070,China;2Hubei Public Security Department,Wuhan 430034,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-01-25 Published:2007-01-25

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Abstract: Based on the nucleotide sequence of JEV SA14-14-2 strain，a pair of primers was designed．The PrM gene of JEV was cloned into general transfer vector pPgG-uni. The sequence analysis showed that the PrM genes of SAl4 and SA14-14-2 strains had 100% homologyA cotransfection experiment was carried out with the purified pPgGPrM and the genome of TK-/gG-/LacZ+ mutant of pseudorabies virus Ea strain in PK-15 cells. By plaque purification and PCR detection, the recombinant virus TK-/gG-/PrM+ was purified. This recombinant virus strain can be used for the study of duel-valence vaccine of JEV and PRV.