Abstract: The objective of the present study was to construct a cDNA library for male adult of Ascaris suum with SMART(switching mechanism at 5′end of RNA transcript) technique using Creator^TM SMART^TM cDNA library construction kit. The total RNA was extracted from A. suum male adult using TriPure isolation reagent and mRNA was purified using Poly(A)Purist^TM kit. Single-strand cDNA was synthesized using PowerScript^TM reverse transcriptase, and then doublestrand cDNA was synthesized and amplified by longdistance PCR (LD-PCR). The PCR products were digested by proteinase K and purified. After digestion with SfiⅠ and size fractionation using CHROMA SPIN-400TM columns, SMART cDNA was ligated to the Sfi I-digested, dephosphorylated pDNR-LIB vector. The ligation mixture was transformed into E.coli DH5α by electroporation. The constructed cDNA library contained 7.26×10⁵ independent clones. The recombination rate was 96.7%. The average cDNA insert size was 1 kb. After the library was amplified, its capacity was 6.359×10⁹ cfu/mL. A cDNA library for A. suum male adult was successfully constructed using SMART technology, which provides foundation for the screening and isolation of male-specific genes from A. suum.