Abstract: Cryopreservation technique of biopsied mouse and bovine blastocysys were also studied. As a result,(1) The biopsied mouse embryos were freezed with 10% glycerol and 10% glycol respectively and, as comparison, the biopsied mouse embryos were freezed with both of the freezing solution added 10% polysaccharid and 10%sucrose, the development rate of frozenthawed mouse embryos were 56.9%(259/455), 81.8%(317/390), 84.8%(540/738), 59.5%(308/518) respectively. (2)The development rate of frozenthawed mouse embryos could not be improved when the biopsied embryos were cultured in vitro shortly (about 0-4 hours) after sampling. (3)Being suffocated 2 minutes at -100 ℃--110 ℃ after sampling and fast cryopreservation with frozen solution which include 25% glycerol and 0.25 mol/L sucrose and 20% blood serum, the development rate of muse embryos was 733%, the pregnancy rate of fast frozenthawed bovine embryos after sampling was 57.1% (4/7).