ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2007, Vol. 38 ›› Issue (10): 1115-1119.doi:

• 研究简报 • Previous Articles     Next Articles

Polymorphism Analysis of MHC-DRB1 Gene in Chinese Merino Sheep by PCRRFLP

PENG Lin-ze;SHEN Hong;JIA Bin;YU Zhi-yong;ZENG Xian-cun;
LIU Hong-kun;PENG Bo;CHEN Ming-hui;GUO Yu-qiang   

  1. 1College of Animal Science & Technology ,Shihezi University,Shihezi 832003, China;2Animal Husbandry and Veterinary Station at Regimental Farm 74 in Xinjiang,Yili 835600, China;3Animal Husbandry and Veterinary Station at Agricultural Construction Division 9 in Xinjiang,Tacheng 834600, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-10-25 Published:2007-10-25

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Abstract: The polymorphism of the exon 2 of MHC-DRB1 was investigated by nested PCR-RFLP technique at three loci digested with SacⅠ,Hin1Ⅰand HaeⅢ,respectively, in a total number of 211 individuals of Chinese Merino(Xinjiang Army Wasteland Reclamation) sheep, and genotypic frequencies and allelic frequencies were counted. The results showed that the extensive polymorphisms of the exon 2 of MHC-DRB1 were found at three loci digested with SacⅠ,Hin1Ⅰ and HaeⅢ,respectively, in Chinese Merino sheep, these sites were controlled by two, two and six alleles, respectively. As some base mutations were found in MHCDRB1 locus, and according to the results in this paper, twentyfour alleles were found in Chinese Merino(Xinjiang Army Wasteland Reclamation) sheep. The results of χ2 test showed that genotypes of MHC-DRB1 digested with SacⅠand Hin1Ⅰexcept HaeⅢ（P＜0.01）fitted with Hardy-Weinberg equilibrium(P＞0.05)