ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2008, Vol. 39 ›› Issue (11): 1562-1566.doi:

• 预防兽医 • Previous Articles     Next Articles

Experimental Study on Dogs Immunized with CPV DNA Vaccine, CPV Recombinant Vector Vaccine and CPV Modified Vaccine

XIE Zhi-jing1,2, YANG Song-tao1, XIA Xian-zhu1*, YAN Fang1,3,
ZHAO Zhong-peng1, GAO Yu-wei1, ZOU Xiao-huan1, HUANG Geng1   

  1. 1.The Military Veterinary Institute, Academy of Military of PLA, Changchun 130062, China; 2.College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China; 3.College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-11-24 Published:2008-11-24

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Abstract: To test the immunogenicity of the different CPV vaccines, the dogs were respectively vaccinated with pVCPV-VP2, CAV2/CPV and CPV modified vaccine. The antibody titers against CPV were detected by CPV ELISA, CPV HI and CPV SN. And the proliferation of the PBMCs of the vaccinated dogs was detected by MTT. As a result, both antiCPV ELISA antibody and SN antibody were checked up on in the dogs vaccinated with CAV2/CPV or pVCPV-VP2. AntiCPV HI antibody was discovered in the dogs immunized with CAV2/CPV or CAV2/CPV combined with pVCPV-VP2, but not in the dogs vaccinated with pVCPV-VP2. And the PBMCs of the inoculated dogs obviously proliferated against both CPV and ConA stimulation, respectively. It demonstrated that CAV2/CPV and pVCPV-VP2 induced humoral and cellular immunity against CPV in dogs. The immune levels elicited by CAV2/CPV or CAV2/CPV combined with pVCPV-VP2 were higher than that by pVCPV-VP2, but weaker than that by the CPV modified vaccine. At the same time, CAV2/CPV can also induce specific anti-CAV-2 immunity.