Abstract: The experiment was conducted to clone the C/EBPα gene in Landes geese liver and to check the relative expression levels of C/EBPα mRNA of different groups. In the study, the gene sequence was obtained based on the C/EBPα gene sequence of chicken. The results showed that the C/EBPα gene has a 975 bp open reading frame (ORF) flanked by a 210 bp 5′UTR and a 397 bp 3′UTR. The deduced amino acid sequence has 324 residues, which was a nucleoprotein residing in nucleolus without signal peptide and transmembrane region.The C/EBPα protein contained a carboxyl terminal basic-leucine zipper (bZIP) domain, which was characterized by cysteinerich, doublezinc finger motifs and a distinct homeodomain. Also, the levels of C/EBPα mRNA of different groups were detected by real-time PCR. Compared to the control group, the levels of C/EBPα mRNA of all overfeeding groups increased significantly (P<0.05 or P<0.01).The level of overfeeding supplemented with T3 and betaine group was higher than that of control group (P<0.01) and other overfeeding groups (P<0.05). So it is suggested that the C/EBPα gene may be related with lipid metabolism.