ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2007, Vol. 38 ›› Issue (7): 708-712.doi:

• 预防兽医 • Previous Articles     Next Articles

Establishment and Initial Application of Low Density DNAarray for Diagnosing the Subtypes of SIV

CHEN Hong-jun;HOU Yi-hong;BAI Hua;CHEN Gui-lai;WU Shi-you;YIN Yan-bo;QIAN Yong-hua;TIAN Zhen-yu   

  1. 1.College of Animal Science and Technology,Northwest A & F University, Yangling 712100,China;2.Shandong Oland Institute of Bioengineering,Qingdao 261001,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-07-25 Published:2007-07-25

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Abstract: The hemagglutinin(HA) and neuraminidase(NA) gene of five subtypes influenza A virus were amplified by RT-PCR with primers based on the diversity of HA and NA gene. Meanwhile conserved cDNA fragments of M gene was amplified by RT-PCR with universal primer of influenza A virus. Each fragments were cloned and inserted into pMD18-T to construct recombinant plasmids for DNA probe preparation. Recovered DNA probes were spotted on located sites on nitrocellulose filter to prepare DNAarrays. 217 different samples of area was detected by this DNAarray. The results showed that this DNAarray could identify five subtypes of Influenza A virus in samples and show high sensitivity and specificity. This DNAarray provids a quick, sensitive and highflux method for largescale epidemiology investigation and quarantine of swine influenza virus.