ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2007, Vol. 38 ›› Issue (9): 881-887.doi:

• 遗传繁育 •     Next Articles

Mapping, Tissue Distribution and Polymorphism of Porcine Cellular Retinol Binding Protein Gene 2 (RBP2) and Cellular Retinoic Acid Binding Protein Gene 2 (CRABP2)

GONG Wei-hua;TANG Zhong-lin;YANG Shu-lin;CUI Wen-tao;LI Kui   

  1. 1.The Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Education of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-09-25 Published:2007-09-25

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Abstract: The retinoid (vitamin A and its derivatives) plays a critical role in vision, growth, reproduction, cell differentiation, and embryonic development. Using the INRA IMpRH panel, porcine cellular retinol binding protein gene 2 (RBP2) and cellular retinoic acid binding protein gene 2 (CRABP2) were assigned to porcine chromosome 13 and 4, respectively. The entire coding sequence (CDS) of porcine CRABP2 was amplified using RT-PCR, and deduced amino acid sequence of porcine CRABP2 was compared to human corresponding protein. The mRNA distributions of the two genes in adult Wuzhishan miniature pig tissues (lung, skeletal muscle, spleen, heart, stomach, large intestine, lymph code, small intestine, liver, brain, kidney, and fat) were examined. A total of four SNPs were identified in porcine RBP2 and CRAPB2. Using PCR-RFLP method, the RBP2C117T SNP locus was analyzed in the Laiwu, Wuzhishan, Guizhou, Bama pig breeds, and Tongcheng pig population. Significant associations were found between different genotypes of RBP2-C117T with marbling score (MS.) and muscle shear force (MSF) traits. This SNP may be used as genetic marker in pig breeding and production.