ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2007, Vol. 38 ›› Issue (9): 893-898.doi:

• 遗传繁育 • Previous Articles     Next Articles

Study on Isolating, RH Mapping of Bovine FABGL Gene

MA Yun;GAO Xue; REN Hong-yan;XU Shang-zhong;ZHANG Ying-han;XIN Ya-ping;GAO Shu-xin   

  1. 1 Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100094,China;2College of Animal Science and Technology, Northwest A & F University, Yangling 712100,China;3． College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao 028000,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-09-25 Published:2007-09-25

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Abstract: The blood samples from Chinese Simmental cattle was collected to extracted genomic DNA and the DNA of the bovine FABGL gene was determined and analyzed by homology cloning approach combined with PCR in this study. Sequence analysis and bioinformatics study showed that this genomic DNA contained 1925 nucleotides and 9 exons. And its base composition shows 90% and 84% identity with the corresponding pig and human, respectively. The SUNbRH panel was employed to determined the precise location of FABGL gene. Statistical analysis revealed that the bovine FABGL gene was mapped to the bovine chromosome (BTA) 23 and was closely linked to a framework marker BM47 at a 2-point LOD score of 31.05.