Abstract: The single nucleotide polymorphism (SNP) of growth hormone gene was investigated in various breeds of duck, including Beijing ducks, Xihu mallards, Cherry Valley Meat ducks, Jinding ducks, Shan Partridge ducks, Jingjiang ducks, Shaoxing ducks and Jinyun Partridge ducks. Eight pairs of primers for coding and regulation regions of GH gene were designed based on the database of duck genomic sequence and the SNPs were detected by PCRSSCP method Three SNPs were found among individuals, which were 230(C→G), 244(C→A) in 5′-UTR and 3 701(C→T) in exon 4， respectively. However, the mutation at 3 701 was a silence mutation. And the 3′-UTR represented its own high conservativeness. The analysis results showed that: ⑴At locus of 5′-UTR, the frequency of B allele in Jinding ducks was significantly higher than that in other breeds. ⑵At locus of exon 4, the frequencies of genotypes in different breeds were significantly different, and the frequency of CC genotype in meattype ducks were significantly higher than that in laying-type ducks. According to the results above, we can conclude that these SNPs may have relationship with some productive traits of ducks.