Abstract: This study sequenced partial sequences of mitochondrial DNA Cyt b gene (420 bp) of 42 individuals and D-loop region (910 bp) of 28 individuals in 5 cattle breeds from Sichuan Province. The results showed that there were 5 mutation sites among the partial Cyt b gene and 7 haplotypes, which was divided into two main types. There were 64 mutation sites and 28 haplotypes defined in Dloop region when the 22 published Chinese cattle D-loop sequences aligned together with the data in this study. Haplotype H1~H23 was belonging to Bos. Taurus type while Bos. Indicus type contained haplotypes from H24 to H28. 67.86% individuals were Bos. Taurus type and 32.14% were Bos. Indicus type in Sichuan cattle breeds. It suggested that the Sichuan cattle were mainly origined from Bos. Taurus type and Bos. Indicus type.