ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2007, Vol. 38 ›› Issue (8): 822-826.doi:

• 预防兽医 • Previous Articles     Next Articles

Cloning and Analysis of Replicase Polyprotein-Encoding ORF1 of Transmissible Gastroenteritis Virus SC-Y Strain

SONG Zhen-hui;GUO Wan-zhu;YIN Hua-ping;ZENG Zhi-yong;ZHU Ling; LUO Ai-fang;ZHANG Ying-jun   

  1. 1.Animal Biology Technology Center of Sichuan Agricultural University,Yaan 625014,China;2 Department of Animal Medical Science, Southwest University, Chongqing 402460,China;3 Library of Southwest University, Chongqing 402460,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-08-25 Published:2007-08-25

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Abstract: In this study, the complete sequence of the replicase polyproteinencoding ORF1 of TGEV SC-Y strain was obtained by RT-PCR, the entire nucleotide sequence was determined(GenBank Accession No. DQ390461).Results showed that there were two partially over lapping open reading frames in replicase polyprotein-encoding ORF1 of SC-Y strain , the ORF1a contains 12 053 nucleotides encoding a polypeptide of 4 018 amino acids, the ORF1b contains 8 036 nucleotides encoding 2 678 amino acids. The sequences were compared among different TGEV strains and other coronary viruses. The results showed that the SC-Y has the highest homology with PUR46MAD strain,the ORF1a and ORF1b nucleotide sequences had 995% and 998%, amino acid sequences had 99.2% and 99.8% respectively. The ORF1b is highly conserved among the different coronaviruses.