ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2009, Vol. 40 ›› Issue (12): 1817-1822.doi:

• 研究简报 • Previous Articles     Next Articles

Effects of PreTreatment with Taxol on Vitrification of in VitroMatured Porcine Oocytes

WANG Lei 1,2,3, YANG Shanting 1,2,3, DAI Jianjun 1,2, WU Caifeng 1,2, ZHANG Tingyu 1,2, ZHANG Defu 1,2*
   

  1. 1.Institute of Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; 2. The Research Group of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of Agrigenetics and Breeding, Shanghai 201106, China; 3. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2009-01-16 Revised:1900-01-01 Online:2009-12-24 Published:2009-12-24
  • Contact: ZHANG Defu

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Abstract: The aim of this study was to improve the effect of vitrification of porcine matured oocyte. This study was designed to examine the effect of Taxol pretreatment on vitrification of porcine oocytes matured in vitro by a cryoloop method. The results showed that the vitrified oocytes pretreated with 1.0 μmol·L1 Taxol attained higher normal morphology rate(89.93%) and FDA stain survival rate(83.33%) than the control(79.12% and 70.97%) (P<0.05). But when the concentration of Taxol was increased, it came to weaken the effect of vitrification. The proportions of oocytes with normal morphology and survive rates could reach to 90.21% and 84.13%, when they were treated for 30 minutes before vitrification. So the best parameter was 1.0 μmol·L1 Taxol for 30 minutes before vitrification. Pretreatment with CB or Taxol had a significant positive effect on vitrification of porcine matured oocytes(P<0.05), but there were no significant differences between the two chemical materials(P>0.05).