ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2010, Vol. 41 ›› Issue (1): 33-38.doi:

• 遗传繁育 • Previous Articles     Next Articles

Bioinformatic Analysis of TMEM66 Gene and Construction of TMEM66 Mutant Transgenic Mice

LI Xinyun 1,2, WANG Chenfang 1, REN Hongyan 1, ZHAO Shuhong 2, YANG Shulin 1, CUI Wentao 1, MU Yulian 1, LI Kui 1*   

  1. 1. The Key Laboratory of Domestic Animal Genetic Resources and Germplasm Innovation，Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193,China; 2. Key Laboratory of Animal Heredity and Breeding of Ministry of Education,Huazhong Agricultural University, Wuhan 430070, China
  • Received:2009-01-03 Revised:1900-01-01 Online:2010-01-24 Published:2010-01-24
  • Contact: LI Kui

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Abstract: Transmembrane protein 66 play important roles in cell apoptosis and tumorigenesis. In order to investigate the function of TMEM66 gene, the transgenic mouse model containing the mutant TMEM66 gene was constructed in this study. The transgenic mice were generated by using microinjection method and 312 injected zygotes were implanted into 13 foster mothers. The positive transgenic mice were confirmed by PCR and Southern blotting. Also, the stability of the transgene was confirmed by passage and the way of integration of the transgene was studied using inverse PCR method. In this study, 6 positive individuals among the 55 neonatal pups were found by Southern blotting. Three positive mice were found to be able to transfer the transgene to next generation. This result indicted that the transgene was integrated into host genome stably. Inverse PCR results indicated that the transgenic DNA fragment was integrated into the host genome in a serial multicopy manner. Therefore, the transgenic mice model containing the mutant TMEM66 gene was constructed successfully. This study offered useful model for further investigation of the function of TMEM66 gene.