Abstract: In order to investigate effect of RPL29 gene associated with protein synthesis on skeletal muscle development, the SYBR-Green quantitative real-time PCR (QPCR) method was established to analyze RPL29 gene. The expression change of RPL29 gene was analyzed in pig embryo skeletal muscle using the QPCR method. Based on standard samples with different concentrations, the standard curve “Y=-3.303X+43.077 (determination coefficient, R2=0.999)” was established. The results indicated the method had large test extension (Ct arranged from 13 to 35 cycle number), high amplification efficiency (100%) and single peak with unique PCR product in the melt curve. The Tm value was 86.5 ℃. In this study, the H3F3A gene was used for internal control, the expression change of RPL29 gene was analyzed in embryo skeletal muscle at 33, 65 and 90 days post coitus (dpc) from Tongcheng and Landrace using the QPCR method. We found that RPL29 exhibited the down- and up-regulation pattern in Tongcheng and Landrace pigs, respectively. There was higher expression in Tongcheng than that in Landrace at 33 dpc, and opposite result between two breeds at 90 dpc. No significant difference was found between two breeds at 65 dpc. These results indicated that RPL29 gene have potential association with skeletal muscle development and effect on meat production traits in pigs.