Abstract: Some suspected haemangiomas type of avian leukosis samples were got from one Hyline brown chinken farm in Shandong Province. Six subgroup J avian leukosis viruses (ALVJ) were isolated and identified, among those were strains two virus strains were isolated from parent and grandparent breeding chickens respectively, the other four strains from commercial chickens. The gp85 gene of all the six ALVJ strains were amplified by PCR with a pair of specific primers designed according to the HPRS103 strain. The analysis of sequence of gp85 gene by DNAstar software showed that Commercial strains 03, 04 and 06 were in the same embranchment with Prent02, the rate of homology was between 97.2% and 97.9%, the rate of homology with HPRS103 was between 94.7% and 95.2%; Commercial 05 strain and Grandparent 01 strain were in the same embranchment, the rate of homology with HPRS103 is 98.3%. The rate among four commercial strains is 95.0%99.9%. All above implied that ALVJ infected in eggtype groups partially comes from grandparent breeding chickens or parent breeding chickens through vertical transmission, or from other resources through horizon transmission. The isolation of the six strains from grandparent, patent and commercial layers is the first reported in China, and it lays a good foundation for the research about gene mutation and tumarigenic mechanism.