ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2011, Vol. 42 ›› Issue (9): 1295-1301.doi:

• 预防兽医 • Previous Articles     Next Articles

Investigation of Gene Polymorphism on Mycoplasma ovipneumoniae Isolates from Some Areas of China

XU Jian1,2， CHU Yue-feng1， ZHAO Ping1，GAO Peng-cheng1， HE Ying1， YAN Gen-qiang2， LU Zhong-xin1*

  

  1. 1. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture,Engineering Research Center of Biological Detection of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China;2. College of Animal Science and Technology, Shihezi University, Shihezi 832000, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-09-23 Published:2011-09-23
  • Contact: LU Zhong-xin

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Abstract: To study the epidemiologic characteristics of Mycoplasma ovipneumoniae isolates from some areas of China, the random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) analysis on 26 strains of M. ovipneumoniae were performed and the gene polymorphism was analyzed by the software NTsys2.10e in this study. The results showed that the 26 strains of M. ovipneumoniae were divided into 6 RAPD groups or 6 RFLP groups when the similarity coefficient was 0.70, 18 RAPD groups or 18 RFLP groups when similarity coefficient was 0.90, 25 RAPD groups or 26 RFLP groups when similarity coefficient was 1.00, respectively. The results revealed that M. ovipneumoniae has an obvious gene polymorphism among Chinese isolates. The gene polymorphism of M. ovipneumoniae is highly relevant with the geographical distribution of the strains, and is also relevant with the host origin of strains. This work will provide some insights to study the epidemiology of M. ovipneumoniae and contribute to the development of vaccine against M. ovipneumoniae in China.