ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2011, Vol. 42 ›› Issue (4): 533-537.doi:

• 预防兽医 • Previous Articles     Next Articles

The Comparison of Serologic Relativity and CPE Types of 3 Avian ReovirusStrains Induced Different Diseases

CHEN Shi-long1,2, CHEN Shao-ying1,2*, CHENG Xiao-xia1,2, JIANG Bin1,2, LIN Feng-qiang1,2,WANG Shao1,2, ZHU Xiao-li1,2, LI Zhao-long1,2, ZHANG Shi-zhong1,2   

  1. 1. Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China; 2. Fujian Animal Diseases Control Technology Development Center, Fuzhou 350013, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-04-20 Published:2011-04-20

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Abstract: This study was conducted to compare the antigenic relativity and cytopathic effect (CPE) characteristics of 3 avian reovirus isolates induced different diseases. The serologic relatedness of avian reovirus (ARV) S1133 strain, muscovyduck reovirus (MDRV) MW9710 strain, novel duck reovirus (NDRV) NP01 strain were determined using cross virus-neutralization (VN) tests Two avian and five mammalian cell lines were evaluated for their application in propagating these avian reovirus strains. Relatedness (R) values, determined by cross VN, revealed that three isolates’ antigen had major differences. These strains could be cultivated successfully in Vero, MDEK, CEF, MDEF, ST, AD293 T cell lines and make some different types of CPE to different cell lines. These results indicated that ARV, MDRV and NDRV’ antigen had major differences and did not share same antigenic relationship with each other. Avain reovirus of different disease-type could be distinguished by the types of CPE.