玉米赤霉烯酮对小鼠T淋巴细胞体外活化、增殖的影响

doi:10.11843/j.issn.0366-6964.2017.07.021

Abstract

Abstract:

In order to reveal the mechanism of the immunosuppressive effect of Fusarium toxin zearalenone (ZEA), this experiment had studied the effects of ZEA on the activation and proliferation of mouse T lymphocytes stimulated by concanavalin A (Con A) in vitro. After being treated with different concentrations of ZEA (0, 10, 20, 40 μmol·L^-1), CCK-8 method was used to detect the proliferation of T lymphocytes. At the same time, the expression levels of the early activation antigen CD69 and metaphase activation antigen CD25 were evaluated by flow cytometry. Results were as follows:Cells were cultured for 48, 72, 96 h, compared with group of cell blank, the group of Con A had obvious proliferating phenomenon (P <0.01). Compared with the group of Con A only, group of ZEA 10 μmoL·L^-1 had an obvious phenomenon of Inhibition of proliferation (P <0.05). When ZEA concentration was 20, 40 μmol·L^-1, the proliferation of T lymphocytes was further inhibited. The proliferation of T lymphocytes stimulated by Con A decreased obviously with the increased concentration of the ZEA which showed in a dose-dependent manner (P <0.01). Cells were stimulated by Con A for 6 or 30 hours. Compared with group of cell blank, the group of Con A had obvious activated phenomenon (P <0.01). Compared with the group of Con A only, group of ZEA 10 μmol·L^-1 had an obvious phenomenon of Inhibition of activation (P <0.01). When ZEA concentration was 20, 40 μmol·L^-1, the expression of CD69 and CD25 was further inhibited. The expression levels of the early activation antigen CD69 and metaphase activation antigen CD25 were significantly inhibited after being treated with the ZEA which showed in a dose-dependent manner (P <0.01). The immunosuppressive effect of ZEA to animals is related to its directly inhibition to the activation and proliferation of T lymphocytes in mouse.

CLC Number:

S859.87

CAI Guo-dong, SUN Kai, XIANG Zi-lai, WANG Ling, ZOU Hui, GU Jian-hong, YUAN Yan, LIU Xue-zhong, LIU Zong-ping, BIAN Jian-chun. The Effects of Zearalenone on the Activation and Proliferation of Mouse T-lymphocytesin vitro[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(7): 1357-1364.

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The Effects of Zearalenone on the Activation and Proliferation of Mouse T-lymphocytesin vitro

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