水牛卵母细胞GV/MⅡ期对应卵丘细胞miRNA差异表达分析

doi:10.11843/j.issn.0366-6964.2017.05.007

Abstract

Abstract:

The aim of this study was to establish the miRNA database of cumulus cells in GV/M Ⅱ stages of buffalo oocytes during maturation in vitro, and then analyze the differential-expression miRNAs of cumulus cells between GV and M Ⅱ stages of oocytes to provide scientific basis for further understanding the important role of cumulus cells in buffalo oocytes maturation. Total RNAs of cumulus cells in GV/MⅡstages were extracted, respectively, and subjected to small RNA sequencing by Solexa sequencing, the miRNA data were then analyzed through bioinformatics. The results indicated that the miRNA expression profiles in GV/MⅡcumulus cells were successfully constructed. There were 24 up-regulated and 45 down-regulated miRNAs in cumulus cells in MⅡ stage compared with those in GV stage. The expression profiles of 8 randomly selected differential-expression miRNAs examined by qRT-PCR was agreement with those by RNA sequencing. Total of 276 enriched signaling pathways related to the miRNA of the cumulus cells were analyzed by miRNA target gene prediction and KEGG enriched pathways. The top 20 enriched signaling pathways were summarized and we found that the differential-expression miRNAs played roles in cumulus mainly through cell proliferation and apoptosis, material meta-bolism, cell junction as well as other unknown pathways.

CLC Number:

S823.8⁺3.2

SHEN Peng-lei, QIN Xi-ling, YIN Qian, GUO Zhen-wei, DENG Kai, DU Shan-shan, YIN Na, RUAN Zi-yun, SHI De-shun, LU Feng-hua. Characterization of the Expression of miRNAs in Cumulus Cells during GV/MⅡ Stages of Buffalo Oocyte[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 836-845.

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Characterization of the Expression of miRNAs in Cumulus Cells during GV/MⅡ Stages of Buffalo Oocyte

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