ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (3): 581-586.doi: 10.11843/j.issn.0366-6964.2016.03.022

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Morphological and Distribution Characteristics of Villous M Cells in the Ileum of Newborn Piglets and the Association with PEDV Infection

LI Ren-feng1,2,4,TIAN Xiang-qin5,QIAO Song-lin2* ,GUO Jun-qing2,ZHOU En-min4,ZHANG Gai-ping3*   

  1. (1.College of Veterinary Medicine,Henan Institute of Science and Technology,Xinxiang 453003,China;2.Key Laboratory of Animal Immunology of the Ministry of Agriculture,Key Laboratory of Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;3.College of Animal Science and Veterinary Medicine,Henan Agricultural University,Zhengzhou 450002,China; 4.College of Veterinary Medicine,Northwest A & F University,Yangling 712100,China;5.Key Open Laboratory for Tissue Regeneration of Henan Universities,Xinxiang Medical University,Xinxiang 453003,China)
  • Received:2015-08-17 Online:2016-03-23 Published:2016-03-23

Abstract:

The aim of this study was to identify the presence of villous M cells in the ileum of newborn piglets and determine the distribution pattern,morphological characteristics and the association with PEDV infection by immunofluorescence assay(IFA),immunohistochemistry(IHC) and morphological methods.IFA showed a gradually decreased density of villous M cells from lower crypt epithelium to upper villous epithelium.The villous M cells showed typical morphological characteristics of M cells by electron microscope.After infected with PEDV,villous M cells in the ileum exhibited pathological changes obviously.The virus particles of PEDV were observed on the apical membrane,in the cytoplasm,around the nucleus and subepithelial of villous M cells by immunohistochemical staining of PEDV S protein,which were not observed in its adjacent epithelial cells.This study demonstrated the presence of villous M cells in the ileum of newborn piglets and determine the distribution pattern,morphological characteristics and the association with PEDV infection,suggesting the villous M cells in the ileum may be an important entry for PEDV invasion.

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