梅花鹿致敏与休眠鹿茸干细胞差异蛋白表达的2D-DIGE分析

doi:10.11843/j.issn.0366-6964.2016.01.013

Abstract

Abstract:

The objective of this study was to screen，identify and analyze the differentially expressed proteins in the potentiated and dormant antler stem cells in sika deer (Cervus nippon)，and then to shed lights on the molecular mechanisms underlying antler regeneration.The two-dimensional fluorescence of gel electrophoresis (2D-DIGE) was used to separate the protein spots；Differentially expressed protein spots were selected by DeCyder 2D (version 7.2)；Matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF-MS) was carried out to obtain peptide mass fingerprinting，Mascot software was used to search the matched proteins in the NCBInr database；PANTHER (Protein Analysis Through Evolutionary Relationships) and REACTOME analysis were performed to further explore the involved signal pathways about these identified proteins.The proteomic profile of the potentiated antler stem cells compared with the dormant antler stem cells was explored by 2D-DIGE.One hundred fifty-nine protein spots with more than 1.1-fold changes and less than -1.1-fold changes and P values less than 0.05 differentially expressed by the potentiated over the dormant antler stem cells，including 110 up-regulated and 49 down-regulated protein spots.Multiple markers were obtained by extended data analysis(EDA) module.MALDI-TOF-MS identified 84 differentially expressed protein spots and 48 of them which came from 27 kinds of proteins were positive.There is a significant difference at proteomic level between the potentiated and the dormant antler stem cells，and some identified proteins which are involved in multiple functional categories might be related to antler regeneration.Therefore，antler regeneration is a process from the dormant to the potentiated states in antler stem cells，which is regulated by multiple proteins and a complicated signal network.

CLC Number:

S825
S813.3

DONG Zhen，WANG Quan-wei，LIU Zhen，SUN Hong-mei，LI Chun-yi. Analysis of Differentially Expressed Proteins in the Potentiated and Dormant Antler Stem Cells through 2D-DIGE[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(1): 91-104.

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Analysis of Differentially Expressed Proteins in the Potentiated and Dormant Antler Stem Cells through 2D-DIGE

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