牦牛卵巢小RNA高通量测序及生物信息学分析

doi:10.11843/j.issn.0366-6964.2016.01.008

Abstract

Abstract:

The small RNA transcriptome profile of yak ovary was described by Solexa deep sequencing，and provided the basic data for future study on yak breeding performance.In this study，yak ovaries were used for building small RNAs library，and then Solexa sequencing and bioinformatics were applied to analysis.Meanwhile，the expression of selected miRNAs was validated by RT-qPCR.After Solexa sequencing，a total of 12 126 208 clean reads were obtained from the ovary library，which contained 212 757 distinct sequences.Alignment analysis showed that 7 383 536 (60.89%) of total sequences and 80 981 (38.06%) distinct sequences were mapped to the yak reference genome.The results of differential expression analysis showed that 56 small RNAs were up-regulated and 33 small RNAs were down-regulated in yak ovary compared to the cattle counterparts.Among these RNA，miR-135a and miR-2316 had the largest fold-change.The expression of 6 selected miRNAs in yak ovary tissues obtained by RT-qPCR was consistent with the Solexa sequencing results.Furthermore，5 novel small RNAs were predicted after compared to the yak EST sequence information.All above suggested the small RNAs transcriptome profile of yak ovary was successfully described，and our results confirmed that Solexa sequencing technology has a great advantage in facilitating small RNA information and mining new miRNAs，and provide valuable small RNA data for further facilitate the yak genetic breeding and study the adaptation and resistance to high altitude and other related fields of highland animal.

CLC Number:

S823.8⁺5.2

XIONG Xian-rong，LAN Dao-liang，LI Jian，ZI Xiang-dong，LIN Ya-qiu，MA Li. Solexa Sequencing of Small RNAs in Yak (Bos grunniens) Ovaries and Bioinformatics Analysis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(1): 55-63.

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Solexa Sequencing of Small RNAs in Yak (Bos grunniens) Ovaries and Bioinformatics Analysis

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