ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (1): 16-24.doi: 10.11843/j.issn.0366-6964.2016.01.003

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Preparation and Study of CuZnSOD Gene Transgenic Pigs in F1 Generation

WANG Shou-dong1,2,FANG Guo-feng1,2,ZENG Yong-qing1,2* ,CHEN Wei1,2,LI Chuan-hao1,2,WANG Yan-dong1,2   

  1. (1.College of Animal Science and Veterinary Medicine,Shandong Agricultural University,Taian 271000,China;2.Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention,Taian 271000,China)
  • Received:2015-01-12 Online:2016-01-23 Published:2016-01-23

Abstract:

The aim of this study was to further research the mechanism of how CuZnSOD gene effect the meat quality by expression and regulation,and explore the possibility to generate transgenic pigs by sperm carrier method mediated by magnetic nanoparticles.F1 generation individuals were obtained by artificial insemination on the basis of the recombinant vector incubated with magnetic nanoparticles and semen,respectively.F1 individuals were detected by PCR,qRT-PCR and Western blot.Exogenous gene copy number in the 4 slaughtered transgenic positive pigs were estimated by absolute qPCR.Individuals from positive and negative groups were selected at the weight of about 100 kg and slaughtered to determine the meat quality and the antioxidant properties.The results showed that:The positive rate was 30.23% in F1 individuals.Compared with the negative group,the CuZnSOD expression at RNA and protein levels in 5 tissues were up-regulated in positive group.The standard curve in this study was:ΔCt=-3.121 6 lgN+17.281(R2=0.992 1,P<0.001).The exogenous gene copy number in the 4 positive pigs was 25.89,80.61,106.69 and 83.03,respectively.The results also showed that:Compared with the negative group,the positive group was significantly increased in muscle T-SOD1d and T-SOD2d activity(P<0.05),but decreased in muscle drip loss,the content of MDA1d and MDA2d(P<0.05),respectively.The results indicated that the overexpression of CuZnSOD gene could elevate the antioxidant properties,reduce the drip loss and further improve the meat quality.Meanwhile,our results proved that transgenic animals could be generated by sperm carrier method mediated by magnetic nanoparticles.

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