牛结蛋白基因启动子的克隆及功能的初步分析

doi:10.11843/j.issn.0366-6964.2014.01.008

Abstract

Abstract:

This study aimed to clone bovine desmin gene promoter fragments of different lengths,and analyze its function.The bovine desmin gene promoter deletion sequences of different lengths were amplified by PCR,pGL3-desminpro dual luciferase expression vector transfected to skeletal muscle satellite cells and bovine fetal fibroblasts for activity detection was constructed.Meanwhile bovine MyoG and α-actin genes promoter expression vectors were transfected in order to compare the desmin,MyoG and α-actin genes promoter expression activity.The results showed that bovine desmin gene in the range of 132-2 106 bp promoter could start the expression of dual luciferase reporter gene at different degrees in bovine skeletal muscle satellite cells,and had muscle-specific.300 bp desmin gene promoter had the highest activity and higher than that of MyoG and α-actin genes promoter activity in bovine.Thus the 300 bp desmin gene promoter activity was higher and was adaptive to the muscle-specific promoter,which has laid an important foundation for the production of transgenic bovine.

CLC Number:

S823
Q523.8

DU Wei,LI Shu-feng,TONG Hui-li,YANG Cui-cui,LIU Dan,YAN Yun-qin. Cloning and Functional Analysis of Bovine Desmin Gene Promoter[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(1): 56-61.

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Cloning and Functional Analysis of Bovine Desmin Gene Promoter

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