保种场涪陵水牛及西南地区水牛品种间遗传结构与ROH分析

doi:10.11843/j.issn.0366-6964.2024.05.017

畜牧兽医学报 ›› 2024, Vol. 55 ›› Issue (5): 1989-1998.doi: 10.11843/j.issn.0366-6964.2024.05.017

保种场涪陵水牛及西南地区水牛品种间遗传结构与ROH分析

屠芸^1,2, 曾雅楠^1,2, 张蒸豪^1,2, 洪瑞^1,2, 王震³, 吴平⁴, 周泽洋^1,2, 叶艺茹^1,2, 杜亚楠^1,2, 左福元^1,2*, 张龚炜^1,2*

1. 西南大学动物科学技术学院, 荣昌 402460;
2. 重庆市肉牛工程技术研究中心, 荣昌 402460;
3. 重庆市畜牧技术推广总站, 重庆 401121;
4. 重庆市南川区畜牧兽医渔业中心, 南川 408400

收稿日期:2023-11-20 出版日期:2024-05-23 发布日期:2024-05-27
通讯作者: 张龚炜，主要从事动物遗传育种研究，E-mail:zgw-vip@163.com ；左福元，主要从事牛的遗传改良及反刍动物营养研究，E-mail:zfuyuan@163.com
作者简介:屠芸(1997-)，女，江苏连云港人，硕士生，主要从事动物遗传育种研究，E-mail:yuntu01@163.com
基金资助:
重庆市技术创新与应用发展专项重点项目(cstc2021jscx-gksbX0012)；重庆市农业种质资源精准鉴定项目(23316)

Genetic Structure and Runs of Homozygosity Analysis of Fuling Buffalo and Southwest Buffalo Breeds

TU Yun^1,2, ZENG Yanan^1,2, ZHANG Zhenghao^1,2, HONG Rui^1,2, WANG Zhen³, WU Ping⁴, ZHOU Zeyang^1,2, YE Yiru^1,2, DU Yanan^1,2, ZUO Fuyuan^1,2*, ZHANG Gongwei^1,2*

1. College of Animal Science and Technology, Southwest University, Rongchang 402460, China;
2. Beef Cattle Engineering and Technology Research Center of Chongqing, Rongchang 402460, China;
3. Chongqing Animal Husbandry Technology Popularization Station, Chongqing 401121, China;
4. Chongqing Nanchuan Animal Husbandry, Veterinary and Fishery Center, Nanchuan 408400, China

Received:2023-11-20 Online:2024-05-23 Published:2024-05-27

摘要/Abstract

摘要： 旨在对保种场涪陵水牛及西南地区其他沼泽型水牛品种进行群体遗传结构和连续纯合片段(ROH)分析，为涪陵水牛保种与遗传改良提供理论依据。本研究对保种场26头涪陵水牛以及1头杂交水牛(FM_HY)进行全基因组重测序，从NCBI下载了68头西南地区沼泽型水牛和7头河流型水牛的基因组重测序数据，基于所有的基因组数据获得的高质量单核苷酸多态性(SNPs)位点进行遗传结构、ROH以及近交系数的分析。全基因组重测序结果显示，涪陵水牛平均测序深度为10×，质量控制后共鉴定出14 326 437个SNPs。群体遗传结构分析显示，保种场内涪陵水牛与毗邻区域的宜宾水牛、贵州水牛、贵州白水牛、盐津水牛间具有明显的遗传结构差异，德宏水牛和滇东南水牛与上述5个品种区别均明显；当分群数量为K=6时，涪陵水牛可划分为2个类群，与毗邻区域水牛品种相比具有一类独特的群体结构。涪陵水牛、宜宾水牛、贵州水牛这3个品种间共检测到2 722个ROHs，其中涪陵水牛检测到1 593个ROHs，且1~2 Mb的短ROH片段占比达78.15%；仅在涪陵水牛群体中检测到8个大于16 Mb的长ROH片段，最长为24.56 Mb。基于ROH计算的保种场涪陵水牛平均近交系数为0.056 8，其中7头个体近交系数较高(0.062 5~0.125 0)；贵州水牛和宜宾水牛的平均近交系数分别为0.034 6和0.041 0。综上，涪陵水牛保种群可分为2个类群，与毗邻区域水牛品种相比具有一类独特的群体结构；保种场内7头涪陵水牛个体存在近交风险，迫切需要加强涪陵水牛的资源保护与利用工作。

关键词: 涪陵水牛, 群体遗传结构, SNP, ROH, 近交系数

Abstract: This study aimed to analyze the population genetic structure and runs of homozygosity (ROH) of Fuling buffalo and other swamp buffalo breeds in southwest China, and provide the theoretical basis for conservation and genetic improvement of Fuling buffalo. In this study, 26 Fuling buffaloes and one hybrid buffalo (FM_HY) were sequenced using whole-genome resequencing technology. The genome resequencing data of 68 swamp buffalo individuals in southwest China and 7 river buffalo individuals were downloaded from the NCBI database. All the genome data were used to obtain high-quality single nucleotide polymorphisms (SNPs) and to analyze the population genetic structure, ROH and inbreeding coefficient. The whole-genome resequencing results showed that the average sequencing depth of Fuling buffalo was 10×, and a total of 14 326 437 SNPs were identified after quality control. The population genetic structure analysis showed that there were significant differences between the Fuling buffalo and the neighboring areas swamp buffalo breeds including Yibin buffalo, Guizhou buffalo, Guizhou White buffalo and Yanjin buffalo. Dehong buffalo and Diandongnan buffalo were significantly different from the above 5 breeds. When the number of clusters was K=6, Fuling buffalo was divided into two groups, which had a unique population structure compared to the neighboring areas buffalo breeds. A total of 2 722 ROHs were detected in Fuling buffalo, Yibin buffalo and Guizhou buffalo. The 1 593 ROHs were identified in Fuling buffalo, and the 78.15% proportion of ROH was the short ROH with 1-2 Mb. Eight long ROH fragments>16 Mb were only detected in Fuling buffalo population, the longest being 24.56 Mb. Based on ROH, the average inbreeding coefficient of Fuling buffalo was 0.056 8, of which 7 individuals had higher inbreeding coefficient in 0.062 5-0.125 0. The average inbreeding coefficients of Guizhou buffalo and Yibin buffalo were 0.034 6 and 0.041 0, respectively. In conclusion, the population of Fuling buffalo in the conservation farm can be divided into two groups, which have a unique population structure compared to the other neighboring areas buffalo breeds, and 7 Fuling buffalo individuals in the conservation farm have inbreeding risk, so it is urgent to strengthen the protection and utilization of Fuling buffalo resources.

Key words: Fuling buffalo, population genetic structure, SNP, ROH, inbreeding coefficient

中图分类号:

S823.83

屠芸, 曾雅楠, 张蒸豪, 洪瑞, 王震, 吴平, 周泽洋, 叶艺茹, 杜亚楠, 左福元, 张龚炜. 保种场涪陵水牛及西南地区水牛品种间遗传结构与ROH分析[J]. 畜牧兽医学报, 2024, 55(5): 1989-1998.

TU Yun, ZENG Yanan, ZHANG Zhenghao, HONG Rui, WANG Zhen, WU Ping, ZHOU Zeyang, YE Yiru, DU Yanan, ZUO Fuyuan, ZHANG Gongwei. Genetic Structure and Runs of Homozygosity Analysis of Fuling Buffalo and Southwest Buffalo Breeds[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 1989-1998.

参考文献

[1] 国家畜禽遗传资源委员会办公室. 关于公布《国家畜禽遗传资源品种名录》的通知[J]. 中华人民共和国农业农村部公报, 2020(6): 36.
Office of Animal Genetic Resources Committee. Notice on National Breed List of Animal Genetic Resources[J]. Gazette of the Ministry of Agriculture and Rural Affairs of the People’s Republic of China, 2020(6): 36.(in Chinese)
[2] 《重庆市畜禽遗传资源志》编写组. 重庆市畜禽遗传资源志[M]. 重庆: 重庆出版社, 2013:65.
Compilation Group of Chongqing Animal Genetic Resources. Animal genetic resources in Chongqing[M]. Chongqing: Chongqing Press, 2013: 65.(in Chinese)
[3] 涪陵水牛育种概况[J].四川畜牧兽医,1976(2):42-46.
General situation of Fuling buffalo breeding[J].Sichuan Animal & Veterinary Sciences,1976(2):42-46.(in Chinese)
[4] 姚仕顺,王德绪.摩拉水牛与涪陵水牛杂交效果初报[J].四川畜牧兽医,1981(4):1-4.
YAO S S,WANG D X.Preliminary report on hybridization effect between Murrah buffalo and Fuling buffalo[J].Sichuan Animal & Veterinary Sciences,1981(4):1-4.(in Chinese)
[5] 田有庆,唐玉华,郭春华,等.涪陵水牛染色体组型分析[J].四川畜牧兽医,1984(4):6-8.
TIAN Y Q,TANG Y H,GUO C H,et al.Chromosome karyotype analysis of Fuling buffalo[J].Sichuan Animal & Veterinary Sciences, 1984(4):6-8.(in Chinese)
[6] 史荣仙,左福元,董学虎.四川水牛血液蛋白多态性研究[J].四川农业大学学报,1992(1):122-126.
SHI R X,ZUO F Y,DONG X H.Studies on blood protein polymorphism in Sichuan swamp buffaloes[J].Journal of Sichuan Agricultural University,1992(1):122-126.(in Chinese)
[7] 史荣仙,付茂忠,赖松家,等.长江流域水牛血液蛋白多态性研究[J].遗传,1995,17(1):7-11.
SHI R X,FU M Z,LAI S J,et al.Study on blood protein polymorphism of buffalo in Yangtze river basin[J].Hereditas (Beijing),1995,17(1):7-11.(in Chinese)
[8] 赖松家,史荣仙,郑维明.中国水牛血清淀粉酶多态性及型命名研究[J].四川农业大学学报,1995,13(2):203-207.
LAI S J,SHI R X,ZHENG W M.Study on serum amylase polymorphism and genetypes named of the buffaloes in China[J].Journal of Sichuan Agricultural University,1995,13(2):203-207.(in Chinese)
[9] 齐国强,昝林森,张桂香,等.中国部分地方水牛品种mtDNA D-loop区遗传多样性与起源研究[J].畜牧兽医学报,2008,39(1):7-11.
QI G Q,ZAN L S,ZHANG G X,et al.Mitochondrial DNA D-loop genetic diversity and origin of some Chinese domestic buffalo breeds[J].Acta Veterinaria et Zootechnica Sinica,2008,39(1):7-11.(in Chinese)
[10] 谢文美,苏锐,李明晖,等.涪陵水牛mtDNA D-loop区遗传多样性研究[J].西北农业学报,2008,17(5):56-60.
XIE W M,SU R,LI M H,et al.Study on mtDNA D-loop genetic diversity in Fuling swamp buffalo[J].Acta Agriculturae Boreali-Occidentalis Sinica,2008,17(5):56-60.(in Chinese)
[11] LOPEZ B I M,AN N,SRIKANTH K,et al.Genomic prediction based on SNP functional annotation using imputed whole-genome sequence data in Korean Hanwoo cattle[J].Front Genet,2021,11:603822.
[12] DEMIR E,MORAV AČG ÍKOVÁ N,KARSLI T,et al.Future perspective of NGS data for evaluation of population genetic structure in Turkish cattle[J].Acta Fytotechn Zootechn,2022,25(2):117-121.
[13] WU F,SUN H,LU S X,et al.Genetic diversity and selection signatures within Diannan small-ear pigs revealed by next-generation sequencing[J].Front Genet,2020,11:733.
[14] CEBALLOS F C,JOSHI P K,CLARK D W,et al.Runs of homozygosity:windows into population history and trait architecture[J].Nat Rev Genet,2018,19(4):220-234.
[15] ONZIMA R B,UPADHYAY M R,DOEKES H P,et al.Genome-wide characterization of selection signatures and runs of homozygosity in Ugandan goat breeds[J].Front Genet,2018,9:318.
[16] SHI L Y,WANG L G,LIU J X,et al.Estimation of inbreeding and identification of regions under heavy selection based on runs of homozygosity in a Large White pig population[J].J Anim Sci Biotechnol,2020,11(1):46.
[17] MARRAS G,GASPA G,SORBOLINI S,et al.Analysis of runs of homozygosity and their relationship with inbreeding in five cattle breeds farmed in Italy[J].Anim Genet,2015,46(2):110-121.
[18] YANG J,LEE S H,GODDARD M E,et al.GCTA:a tool for genome-wide complex trait analysis[J].Am J Hum Genet,2011, 88(1):76-82.
[19] ALEXANDER D H, LANGE K. Enhancements to the ADMIXTURE algorithm for individual ancestry estimation[J]. BMC Bioinformatics, 2011, 12: 246.
[20] 赵真坚,王书杰,陈栋,等.基于低深度全基因组测序分析内江猪群体结构和遗传多样性[J].畜牧兽医学报,2023,54(6):2297-2307.
ZHAO Z J,WANG S J,CHEN D,et al.Population structure and genetic diversity analysis of Neijiang pigs based on low-coverage whole genome sequencing[J].Acta Veterinaria et Zootechnica Sinica,2023,54(6):2297-2307.(in Chinese)
[21] 陶璇,杨雪梅,梁艳,等.基于SNP芯片的丫杈猪保种群体遗传结构研究[J].畜牧兽医学报,2023,54(6):2308-2319.
TAO X,YANG X M,LIANG Y,et al.Analysis of genetic structure of conservation population in Yacha pig based on SNP chip[J].Acta Veterinaria et Zootechnica Sinica,2023,54(6):2308-2319.(in Chinese)
[22] 胡紫平,王立刚,宗文成,等.基于基因组SNP和ROH的剑白香猪群体遗传结构解析[J].畜牧兽医学报,2023,54(10):4117-4125.
HU Z P,WANG L G,ZONG W C,et al.Genetic structure analysis of Jianbai Xiang pig population based on genomic SNP and ROH[J].Acta Veterinaria et Zootechnica Sinica,2023,54(10):4117-4125.(in Chinese)
[23] 齐丽娜,陆雪林,杨凯旋,等.基于SNP芯片分析新浦东鸡的遗传多样性和遗传结构[J].畜牧兽医学报,2023,54(12):4962-4971.
QI L N,LU X L,YANG K X,et al.Analysis of genetic diversity and genetic structure of new Pudong chicken based on SNP chips[J].Acta Veterinaria et Zootechnica Sinica,2023,54(12):4962-4971.(in Chinese)
[24] 武艳平,魏岳,康昭风,等.基于全基因组SNP分析8个地方鸡品种的遗传多样性[J].畜牧兽医学报,2022,53(2):646-653.
WU Y P,WEI Y,KANG Z F,et al.Genetic diversity analysis of 8 local chicken breeds based on whole genome SNP[J].Acta Veterinaria et Zootechnica Sinica,2022,53(2):646-653.(in Chinese)
[25] 高超群,曹然然,杜文苹,等.基于全基因组SNP标记分析中国地方鸡品种的遗传多样性和种群结构[J].畜牧兽医学报,2023,54(2):554-562.
GAO C Q,CAO R R,DU W P,et al.Genetic diversity and population structure analysis of Chinese native chicken breeds using genome-wide SNPs[J].Acta Veterinaria et Zootechnica Sinica,2023,54(2):554-562.(in Chinese)
[26] 刘宏祥,沈永杰,张丽华,等.基于简化基因组测序的娄门鸭遗传多样性评价[J].畜牧兽医学报,2022,53(6):1735-1748.
LIU H X,SHEN Y J,ZHANG L H,et al.Genetic diversity evaluation of Loumen duck based on reduced-representation genome sequencing[J].Acta Veterinaria et Zootechnica Sinica,2022,53(6):1735-1748.(in Chinese)
[27] 马浩然,张路培,金生云,等.利用高密度SNP芯片评估中国地方肉牛品种基因组亲缘关系[J].畜牧兽医学报, 2023,54(10):4174-4185.
MA H R,ZHANG L P,JIN S Y,et al.Assessment of the genomic relationships for Chinese indigenous beef cattle using high-density SNP chip[J].Acta Veterinaria et Zootechnica Sinica,2023,54(10):4174-4185.(in Chinese)
[28] 马克岩,韩金涛,白雅琴,等.基于简化基因组测序的永登七山羊遗传多样性分析[J].畜牧兽医学报,2023,54(5):1939-1950.
MA K Y,HAN J T,BAI Y Q,et al.Genetic diversity analysis of Yongdeng Qishan sheep based on specific-locus amplified fragment sequencing[J].Acta Veterinaria et Zootechnica Sinica,2023,54(5):1939-1950.(in Chinese)
[29] 张任豹,周东辉,周李生,等.基于70 K SNP芯片分析济宁青山羊保种群体的遗传结构[J].畜牧兽医学报,2023,54(7):2836-2847.
ZHANG R B,ZHOU D H,ZHOU L S,et al.Analysis of genetic structure of conservation population in Jining gray goats based on 70 K SNP chip[J].Acta Veterinaria et Zootechnica Sinica,2023,54(7):2836-2847.(in Chinese)
[30] 李隐侠,牙生江·那斯尔,赛里克·都曼,等.SNP芯片评估柯尔克孜羊群体遗传多样性和遗传结构[J].畜牧兽医学报, 2023,54(2):572-583.
LI Y X,NASIER Y,DUMAN S,et al.Evaluation of genetic diversity and genetic structure in Kirgiz sheep population based on SNPs chip[J].Acta Veterinaria et Zootechnica Sinica,2023,54(2):572-583.(in Chinese)
[31] SUN T,SHEN J F,ACHILLI A,et al.Genomic analyses reveal distinct genetic architectures and selective pressures in buffaloes[J]. Gigascience,2020,9(2):giz166.
[32] LUO X E,ZHOU Y,ZHANG B,et al.Understanding divergent domestication traits from the whole-genome sequencing of swamp- and river-buffalo populations[J].Natl Sci Rev,2020,7(3):686-701.
[33] FEREN AČG AKOVIĆ M,SÖLKNER J,CURIK I.Estimating autozygosity from high-throughput information:effects of SNP density and genotyping errors[J].Genet Sel Evol,2013,45(1):42.
[34] MASTRANGELO S,CIANI E,MARSAN P A,et al.Conservation status and historical relatedness of Italian cattle breeds[J].Genet Sel Evol,2018,50(1):35.
[35] LIU S H,MA X Y,HASSAN F U,et al.Genome-wide analysis of runs of homozygosity in Italian Mediterranean buffalo[J].J Dairy Sci,2022,105(5):4324-4334.
[36] PURFIELD D C,BERRY D P,MCPARLAND S,et al.Runs of homozygosity and population history in cattle[J].BMC Genet,2012, 13(1):70.
[37] PERIPOLLI E,MUNARI D P,SILVA M V G B,et al.Runs of homozygosity:current knowledge and applications in livestock[J]. Anim Genet,2017,48(3):255-271.
[38] PERIPOLLI E,STAFUZZA N B,MUNARI D P,et al.Assessment of runs of homozygosity islands and estimates of genomic inbreeding in Gyr (Bos indicus) dairy cattle[J].BMC Genomics,2018,19(1):34.
[39] GHOREISHIFAR S M,MORADI-SHAHRBABAK H,FALLAHI M H,et al.Genomic measures of inbreeding coefficients and genome-wide scan for runs of homozygosity islands in Iranian river buffalo,Bubalus bubalis[J].BMC Genet,2020,21(1):16.
[40] RAFIEPOUR M,EBRAHIMIE E,VAHIDI M F,et al.Whole-genome resequencing reveals adaptation prior to the divergence of buffalo subspecies[J].Genome Biol Evol,2021,13(1):evaa231.
[41] NASCIMENTO A V,CARDOSO D F,SANTOS D J A,et al.Inbreeding coefficients and runs of homozygosity islands in Brazilian water buffalo[J].J Dairy Sci,2021,104(2):1917-1927.
[42] MILLS R E,WALTER K,STEWART C,et al.Mapping copy number variation by population-scale genome sequencing[J].Nature, 2011,470(7332):59-65.
[43] STRILLACCI M G,MORADI-SHAHRBABAK H,DAVOUDI P,et al.A genome-wide scan of copy number variants in three Iranian indigenous river buffaloes[J].BMC Genomics,2021,22(1):305.
[44] ZHANG X F,CHEN N B,CHEN H,et al.Comparative analyses of copy number variations between swamp and river buffalo[J].Gene,2022,830:146509.
[45] YANG L,HAN J Z,DENG T X,et al.Comparative analyses of copy number variations between swamp buffaloes and river buffaloes[J].Anim Genet,2023,54(2):199-206.

保种场涪陵水牛及西南地区水牛品种间遗传结构与ROH分析

Genetic Structure and Runs of Homozygosity Analysis of Fuling Buffalo and Southwest Buffalo Breeds

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