山羊支原体肺炎的CT影像分析

doi:10.11843/j.issn.0366-6964.2021.05.027

畜牧兽医学报 ›› 2021, Vol. 52 ›› Issue (5): 1414-1423.doi: 10.11843/j.issn.0366-6964.2021.05.027

山羊支原体肺炎的CT影像分析

姜敏¹, 姚大伟¹, 田睿², 毛立², 史超颖³, 李文良², 张纹纹², 李基棕², 杨蕾蕾², 孙敏², 杨德吉¹, 刘茂军^2*

1. 南京农业大学动物医学院, 南京 210095;
2. 江苏省农业科学院兽医研究所/农业部兽用生物制品工程技术重点实验室, 南京 210019;
3. 南京艾贝尔动物医学中心, 南京 210046

收稿日期:2020-09-24 出版日期:2021-05-23 发布日期:2021-05-22
通讯作者: 杨德吉,主要从事兽医影像诊断学研究,E-mail:djyang@njau.edu.cn;刘茂军,主要从事动物传染病学研究,E-mail:maojunliu@163.com
作者简介:姜敏(1994-),女,湖南益阳人,硕士生,主要从事动物MRI、CT影像诊断技术研究,E-mail:jiangmin82@vip.qq.com;姚大伟(1979-),男,山东枣庄人,副教授,主要从事兽医影像学研究,E-mial:yaodawei@njau.edu.cn。
基金资助:
国家重点研发计划项目（2017YFD0502200）；中央高校基本科研业务费专项（KYYJ201809）；江苏省第五期“333高层次人才培养工程”科研项目（BRA2019092）

Computer Tomography Images Analyses of Mycoplasma Pneumonia of Goats

JIANG Min¹, YAO Dawei¹, TIAN Rui², MAO Li², SHI Chaoying³, LI Wenliang², ZHANG Wenwen², LI Jizong², YANG Leilei², SUN Min², YANG Deji¹, LIU Maojun^2*

1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;
2. Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210019, China;
3. Nanjing Ai-bi Pet Medical Center, Nanjing 210046, China

Received:2020-09-24 Online:2021-05-23 Published:2021-05-22

摘要/Abstract

摘要： 旨在研究羊感染肺炎支原体后胸部CT影像学特征。将20只山羊分为2组，对照组5只、试验组15只，试验组通过气管注射感染5 mL绵羊肺炎支原体（1×10⁷ CCU·mL^-1）人工诱发山羊支原体肺炎，对照组注射等体积生理盐水。感染后观察两组羊的临床症状，在感染后第0、7、14、21、28天对两组羊胸部进行CT扫查，分析支原体肺炎的CT影像学特征。感染后第29天剖杀羊，观察肺部病理解剖变化。取病变组织制备石蜡切片，观察组织病理学变化。结果显示：试验组山羊感染肺炎支原体后表现出呼吸道感染症状，体温升高，咳嗽，流浆液性或脓性鼻液。胸部CT影像主要表现为片状磨玻璃密度影，网格状阴影，多见双侧肺叶病变，好发于右前叶，以间质性肺炎伴发支气管肺炎为主，其中，重症羊多见空气支气管征，个别羊见胸膜增厚影。对照组临床症状和胸部CT影像在感染前后未见明显差异和异常。综上表明，CT影像诊断技术有利于羊支原体肺炎的早期诊断，并有助于疾病转归的判断。

关键词: 羊, 支原体, 肺炎, 计算机断层扫描

Abstract: The aim of this study was to investigate the characteristics of computer tomography images after Mycoplasma ovipneumoniae infection in goats. Twenty 1-year-old goats were randomly divided into the control group (n=5) and the experimental group (n=15). In the experimental group, mycoplasma pneumonia was artificially induced by intratracheal injection with 5 mL Mycoplasma ovipneumoniae standard strain IK3-3 (1×10⁷ CFU·mL^-1), the control group was injected with equal volume physiological saline. After inoculation, the clinical symptoms of the two groups were observed. At the same time, CT scan was performed on the chest at day 0, 7, 14, 21 and 28 after infection to analyze the characteristics of images. The goats were killed at day 29 after infection to observe the pathological and histopathological changes between the two groups. As a result, after successful infection, the experimental goats expressed obvious clinical symptoms of respiratory diseases, such as high temperatures, cough, serous and purculent nasal discharge. In the experimental group, CT images were mainly characterized by ground glass density shadow and mesh shadow, mostly in bilateral lobar lesions, preferably in the right anterior lobe. Interstitial pneumonia with bronchopneumonia was the main characteristic. In addition, air bronchogram was observed in severe group’s cases and thickened pleural was found in some cases. There was no significant difference before and after infection in normal group goats. It was suggested that CT technology was conducive for the early diagnosis of mycoplasma pneumoniae in goats and helpful to judge the outcomes.

Key words: goat, Mycoplasma ovipneumonia, pneumonia, computed tomography

中图分类号:

S854.7

姜敏, 姚大伟, 田睿, 毛立, 史超颖, 李文良, 张纹纹, 李基棕, 杨蕾蕾, 孙敏, 杨德吉, 刘茂军. 山羊支原体肺炎的CT影像分析[J]. 畜牧兽医学报, 2021, 52(5): 1414-1423.

JIANG Min, YAO Dawei, TIAN Rui, MAO Li, SHI Chaoying, LI Wenliang, ZHANG Wenwen, LI Jizong, YANG Leilei, SUN Min, YANG Deji, LIU Maojun. Computer Tomography Images Analyses of Mycoplasma Pneumonia of Goats[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(5): 1414-1423.

参考文献 15

[1]	张轩, 储岳峰, 逯忠新. 家畜重要支原体病疫苗的研究进展[J]. 中国兽医科学, 2011, 41(12):1314-1320.ZHANG X, CHU Y F, LU Z X. Research advance in vaccines against important mycoplasmal diseases in livestock[J]. Chinese Veterinary Science, 2011, 41(12):1314-1320. (in Chinese)
[2]	张秀坤, 陈小云. 山羊传染性胸膜肺炎研究进展[J]. 中国兽药杂志, 2019, 53(12):66-72.ZHANG X K, CHEN X Y. Progress in the diagnosis of contagious caprine pleuropneumonia[J]. Chinese Journal of Veterinary Drug, 2019, 53(12):66-72. (in Chinese)
[3]	THARWAT M, AL-SOBAYIL F. Ultrasonographic findings in goats with contagious caprine pleuropneumonia caused by Mycoplasma capricolum subsp. capripneumoniae[J]. BMC Vet Res, 2017, 13:263.
[4]	LIN J H, CHEN S P, YEH K S, et al. Mycoplasma hyorhinis in Taiwan:diagnosis and isolation of swine pneumonia pathogen[J]. Vet Microbiol, 2006, 115(1-3):111-116.
[5]	蔡洪贺, 张克宇, 王献忠. 高分辨率CT上树芽征在肺内疾病鉴别诊断中的价值[J]. 中国医学影像技术, 2003, 19(1):54-56.CAI H H, ZHANG K Y, WANG X Z. Value of tree-in-bud sign on high-resolution CT in differential diagnosis of the lung diseases[J]. Chinese Journal of Medical Imaging Technology, 2003, 19(1):54-56. (in Chinese)
[6]	HENNINGER W. Use of computed tomography in the diseased feline thorax[J]. J Small Anim Pract, 2003, 44(2):56-64.
[7]	JOHNSON V S, CORCORAN B M, WOTTON P R, et al. Thoracic high-resolution computed tomographic findings in dogs with canine idiopathic pulmonary fibrosis[J]. J Small Anim Pract, 2005, 46(8):381-388.
[8]	DØNNEM I, EKNÆS M, RANDBY Å T. Energy status, measured by computer tomography (CT)-scanning, and milk quality of dairy goats fed rations with various energy concentrations[J]. Livest Sci, 2011, 142(1-3):235-244.
[9]	LUBBERS B Y, APLEY M D, COETZEE J F, et al. Use of computed tomography to evaluate pathologic changes in the lungs of calves with experimentally induced respiratory tract disease[J]. Am J Vet Res, 2007, 68(11):1259-1264.
[10]	孙喜安. 绵羊肺炎支原体内蒙古地方株的分离鉴定及交叉反应性的测定[D]. 呼和浩特:内蒙古农业大学, 2012:88-120.SUN X A. Isolation and identification of Mycoplasma ovipeneumoniae inner Mongolia local strain and cross reacticity measurement[D]. Hohhot: Inner Mongolia Agricultural University, 2012:88-120. (in Chinese)
[11]	BESSER T E, HIGHLAND M A, BAKER K, et al. Causes of pneumonia epizootics among bighorn sheep, Western United States, 2008-2010[J]. Emerg Infect Dis, 2012, 18(3):406-414.
[12]	RAGHAVAN B, BAVANANTHASIVAM J, KUGADAS A, et al. Effect of vaccination against pneumonia on the survival of bighorn sheep (Ovis canadensis) commingled with carrier animals[J]. Vet Microbiol, 2017, 203:56-61.
[13]	WILCZYŃSKI J R. Immunological analogy between allograft rejection, recurrent abortion and pre-eclampsia-the same basic mechanism?[J]. Hum Immunol, 2006, 67(7):492-511.
[14]	AU J J, WEISMAN D L, STEFANACCI J D, et al. Use of computed tomography for evaluation of lung lesions associated with spontaneous pneumothorax in dogs:12 cases (1999-2002)[J]. J Am Vet Med Assoc, 2006, 228(5):733-737.
[15]	BESSER T E, CASSIRER E F, POTTER K A, et al. Correction:exposure of bighorn sheep to domestic goats colonized with Mycoplasma ovipneumoniae induces sub-lethal pneumonia[J]. PLoS One, 2018, 13(1):e0192006.

山羊支原体肺炎的CT影像分析

Computer Tomography Images Analyses of Mycoplasma Pneumonia of Goats

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献 15

相关文章 15

编辑推荐

Metrics

本文评价

[1]	董书餐, 毛帅翔, 伍翠莹, 李耀坤, 孙宝丽, 郭勇庆, 邓铭, 刘德武, 柳广斌. 雄激素受体抑制剂恩杂鲁胺对山羊卵泡颗粒细胞增殖凋亡的影响[J]. 畜牧兽医学报, 2024, 55(5): 2022-2031.
[2]	罗婷, 韩著, 徐业芬, 蔡林, 索朗斯珠, 徐晋花, 牛家强. 西藏牦牛源牛支原体T10株全基因组测序及其序列分析[J]. 畜牧兽医学报, 2024, 55(5): 2154-2167.
[3]	王吉英, 尹蕊如, 谢星, 王海燕, 刘胡栋, 胡辉, 熊祺琰, 冯志新, 邵国青, 于岩飞. 猪肺炎支原体乳酸脱氢酶在诱导猪支气管上皮细胞凋亡中的作用[J]. 畜牧兽医学报, 2024, 55(5): 2195-2205.
[4]	李秋云, 田芯源, 廖文圣, 张焕容, 任玉鹏, 杨发龙, 朱江江, 向华. SOCS2对山羊鼻甲骨细胞增殖、周期及凋亡的影响[J]. 畜牧兽医学报, 2024, 55(5): 2226-2240.
[5]	李鹏飞, 高桂琴, 周广青, 吴锦艳, 颜新敏, 曹小安, 何继军, 袁莉刚, 尚佑军. 山羊地方性鼻内肿瘤病毒TaqMan荧光定量RT-PCR检测方法的建立及应用[J]. 畜牧兽医学报, 2024, 55(5): 2259-2266.
[6]	彭佩雅, 陈钰焓, 杨龙, 王铭, 赵芮葶, 何俊, 印遇龙, 刘梅. 家畜基因组拷贝数变异研究进展[J]. 畜牧兽医学报, 2024, 55(4): 1356-1369.
[7]	张少华, 王帅, 邹扬, 刘仲藜, 才学鹏. 羊捻转血矛线虫病检测方法研究进展[J]. 畜牧兽医学报, 2024, 55(4): 1499-1510.
[8]	杨杨, 余乾, 刘昱成, 杨华, 赵卓, 王立民, 周平, 杨庆勇, 代蓉. 绵羊MYL基因家族的鉴定与组织表达分析[J]. 畜牧兽医学报, 2024, 55(4): 1551-1564.
[9]	徐俊杰, 张璐通, 王津洁, 陈晓晨, 何伟先, 蔡传江, 褚瑰燕, 杨公社. 基于多组学与网络药理学探究淫羊藿对后备母猪发情的作用[J]. 畜牧兽医学报, 2024, 55(4): 1615-1628.
[10]	罗小芬, 谢晓东, 赵超, 胡茜, 王永璇, 冉芳菲, 胡鹏飞, 文明, 朱二鹏, 程振涛. 牛支原体贵州株黏附相关蛋白的初步鉴定[J]. 畜牧兽医学报, 2024, 55(4): 1672-1683.
[11]	江锦秀, 张靖鹏, 林裕胜, 刘维巍, 胡奇林, 万春和. 基于Hsp70基因的绵羊肺炎支原体TaqMan检测方法的建立及其遗传演化分析[J]. 畜牧兽医学报, 2024, 55(4): 1684-1695.
[12]	杜改梅, 王月, 茅慧华, 雷卫强, 储岳峰, 刘茂军. 绵羊肺炎支原体小鼠感染模型的建立[J]. 畜牧兽医学报, 2024, 55(4): 1728-1737.
[13]	常馨丹, 胡帆, 伍志武, 叶炳森, 刘铁海, 林杰, 贺志雄, 谭支良. 日粮添加高比例过瘤胃脂肪对生长肉用绵羊采食行为的影响[J]. 畜牧兽医学报, 2024, 55(3): 1077-1084.
[14]	黄媛媛, 王佳, 陈嘉瑜, 甘源, 袁厅, 冯志新, 邵国青, 王先炜, 熊祺琰. 猪腹膜间皮细胞的分离培养及初步应用[J]. 畜牧兽医学报, 2024, 55(3): 1249-1256.
[15]	武文英, 夏青, 胡萌婕, 赵逸轩, 王琛, 张宇豪, 郝成武, 贺笋, 郭爱珍, 陈建国, 陈颖钰. 牛支原体兔体攻毒模型的建立[J]. 畜牧兽医学报, 2024, 55(3): 1268-1277.