新疆部分地区马疱疹病毒1型感染的血清学调查

doi:10.11843/j.issn.0366-6964.2021.03.029

摘要/Abstract

摘要： 旨在了解新疆地区马疱疹病毒1型感染的流行现状和特点，本研究于2019—2020年从新疆乌鲁木齐、昌吉州、伊犁州、巴州16处规模化马场收集1 657份马血清样品，采用酶联免疫吸附试验（ELISA）进行抗体检测，对不同地区、品种、性别及年龄马匹的马疱疹病毒1型抗体阳性率进行统计分析。结果显示，乌鲁木齐、伊犁州、昌吉州、巴州马疱疹病毒1型抗体阳性率分别为39.34%、22.56%、90.52%、5.00%；纯血马、混血马、哈萨克马、伊犁马、焉耆马、普氏野马的EHV-1抗体阳性率分别为20.34%、32.93%、44.92%、19.35%、5.00%、90.52%；普氏野马与家马的EHV-1抗体阳性率分别为90.52%和29.32%；家马中新疆本土品种马与引进品种马的EHV-1抗体阳性率分别为31.70%和25.50%；母马与公马的EHV-1抗体阳性率分别为38.84%和22.00%；处于幼年、青年、成年、老年阶段的马匹EHV-1抗体阳性率分别为35.37%、33.00%、34.31%、23.81%。结果提示，新疆乌鲁木齐、伊犁州、昌吉州、巴州规模化养殖场普遍存在EHV-1感染，且不同地区、品种、性别及年龄的马EHV-1感染率差异显著（P<0.05）。本研究为新疆地区EHV-1感染的探讨和防控工作提供调查数据。

关键词: 马疱疹病毒1型, 酶联免疫吸附试验, 血清学调查

Abstract: This study aimed to investigate the epidemic status and characteristics of equid herpesvirus-1(EHV-1) infection in Xinjiang. One thousand six hundred and fifty-seven samples of horse sera were collected from Urumqi, Yili, Changji, Bazhou, from 2019 to 2020. Enzyme-linked immunosorbent assay (ELISA) was performed to detect EHV-1 antibody. Statistical analysis of the positive rate of EHV-1 antibody in different regions, breeds, genders and ages. The detection rate of Urumqi, Yili, Changji and Bazhou were 39.34%, 22.56%, 90.52% and 5.00%; The positive rate of Thoroughbred horse, Half-bred horse, Hasake horse, Yili horse, Yanqi horse, Przewalski's horse were 20.34%, 32.93%, 44.92%, 19.35%, 5.00%, 90.52%, respectively; The positive rate of Przewalski's horse and domestic horse were 90.52% and 29.32%, respectively. The positive rate of local variety and introduced variety were 31.70% and 25.50%; The positive rate of female and male were 38.84% and 22.00%; The positive rate of infancy, youth, adult, old age were 35.37%, 33.00%, 34.31% and 23.81%, respectively. The results indicated that EHV-1 infection was widespread in large-scale stud-farms in Urumqi, Changji, Yili and Baizhou of Xinjiang. The infection rates of EHV-1 in different regions, breeds, genders and ages were significantly different (P<0.05). This study provides survey data for the research and prevention of EHV-1 infection in the region.

Key words: equid herpesvirus-1, enzyme linked immunosorbent assay, sero-epidemiology investigation

中图分类号:

S852.659.1

胡月, 高雁怩, 加尔肯, 车传忠, 王旭蕾, 姜平, 冉多良, 刘建华. 新疆部分地区马疱疹病毒1型感染的血清学调查[J]. 畜牧兽医学报, 2021, 52(3): 845-850.

HU Yue, GAO Yanni, Jarhen, CHE Chuanzhong, WANG Xulei, JIANG Ping, RAN Duoliang, LIU Jianhua. Serological Investigation of Equid Herpesvirus-1 Infection in Some Areas of Xinjiang[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(3): 845-850.

参考文献 21

[1]	OLADUNNI F S, HOROHOV D W, CHAMBERS T M. EHV-1:a constant threat to the horse industry[J]. Front Microbiol, 2019, 10:2668.
[2]	MA G G, AZAB W, OSTERRIEDER N. Equine herpesviruses type 1(EHV-1) and 4(EHV-4)——masters of co-evolution and a constant threat to equids and beyond[J]. Vet Microbiol, 2013, 167(1-2):123-134.
[3]	BRYANT N A, WILKIE G S, RUSSELL C A, et al. Genetic diversity of equine herpesvirus 1 isolated from neurological, abortigenic and respiratory disease outbreaks[J]. Transbound Emerg Dis, 2018, 65(3):817-832.
[4]	OIE. Final Report of the 80th general session[EB/OL]. (2012-05-25)[2020-06-30]. https://www.oie.int/fileadmin/Home/eng/About_us/docs/pdf/A_FR_2012_Public.pdf.
[5]	中华人民共和国农业农村部. 中华人民共和国海关总署第256号公告[EB/OL]. (2020-01-15)[2020-07-03]. http://www.hnxmsyzz.com/zytg/show-2007.html. Ministry of Agriculture of the People' Republic of China. Announcement No. 256 of the general administration of customs of the People's Republic of China[EB/OL]. (2020-01-15)[2020-07-03]. http://www.hnxmsyzz.com/zytg/show-2007.html. (in Chinese)
[6]	罗蔼剑, 张莹, 刁巧虹, 等. 我国马鼻肺炎流行现状[J]. 中国兽医杂志, 2016, 52(11):84-87.LUO G J, ZHANG Y, DIAO Q H, et al. Current status of equine rhino pneumonia in China[J]. Chinese Journal of Veterinary Medicine, 2016, 52(11):84-87. (in Chinese)
[7]	郑小龙, 王群, 孙明君, 等. 马鼻肺炎病毒进化分析及DNA条形码的确定[J]. 中国兽医学报, 2017, 37(2):238-242.ZHENG X L, WANG Q, SUN M J, et al. Evolution analysis on equine herpesvirus and DNA barcodes identification[J]. Chinese Journal of Veterinary Science, 2017, 37(2):238-242. (in Chinese)
[8]	宋焕堂, 孙建华, 杜润慈, 等. 马疱疹病毒抗体间接ELISA检测方法的建立[J]. 畜牧与兽医, 2016, 48(6):1-5.SONG H T, SUN J H, DU R C, et al. Establishment of indirect ELISA for detection of antibodies against equine herpesviruses[J]. Animal Husbandry & Veterinary Medicine, 2016, 48(6):1-5. (in Chinese)
[9]	李娜, 孙建华, 石明, 等. 新疆部分地区马鼻肺炎的血清学调查[J]. 动物医学进展, 2014, 35(9):115-117.LI N, SUN J H, SHI M, et al. Erological investigation of equine rhinopneumonitis in part of regions of Xinjiang[J]. Progress in Veterinary Medicine, 2014, 35(9):115-117. (in Chinese)
[10]	阿依吐拉·肉孜, 阿孜古丽, 肖开提, 等. 新疆地区马鼻肺炎血清学调查[J]. 中国畜牧兽医文摘, 2013, 29(12):82.AYITULA R, AZIGULI, XIAO K T, et al. Serological investigation of equine rhinopneumonia in Xinjiang[J]. Chinese Abstracts of Animal Husbandry and Veterinary Medicine, 2013, 29(12):82. (in Chinese)
[11]	沙燕, 苏战强, 杜润慈, 等. 新疆伊犁地区部分马病血清学调查[J]. 畜牧与兽医, 2016, 48(8):91-94.SHA Y, SU Z Q, DU R C, et al. Serological investigation of some equine diseases in Yili area of Xinjiang[J]. Animal Husbandry & Veterinary Medicine, 2016, 48(8):91-94. (in Chinese)
[12]	杜润慈, 刘建华, 李静, 等. 新疆伊犁部分地区马鼻肺炎血清学调查[J]. 黑龙江畜牧兽医, 2016(11):120-122.DU R C, LIU J H, LI J, et al. Serological survey of equine rhinopneumonitis in parts of Ili prefecture of Xinjiang[J]. Heilongjiang Animal Science and Veterinary Medicine, 2016(11):120-122. (in Chinese)
[13]	袁贝, 李海, 马陈, 等. 新疆伊犁地区马鼻肺炎、马腺疫、马流产沙门菌病的血清学调查[J]. 中国预防兽医学报, 2017, 39(7):540-543.YUAN B, LI H, MA C, et al. Serum epidemiology investigation of Equine rhinopneumonitis, Strangles, Salmonella abortus equi in Yili of Xinjiang[J]. Chinese Journal of Preventive Veterinary Medicine, 2017, 39(7):540-543. (in Chinese)
[14]	王旭蕾. 马疱疹病毒1型gG蛋白间接ELISA方法的建立和gG、gD串联基因的原核表达载体的构建[D]. 乌鲁木齐:新疆农业大学, 2020.WANG X L. Establishment of indirect ELISA method for equine herpesvirus type 1 gG gene and construction of proharyotic expression vector of gG and gD tandem genes[D]. Urumqi:Xinjiang Agricultural University, 2020. (in Chinese)
[15]	陈琳, 王镇山. 普氏野马的回顾与保护现状[J]. 新疆林业, 2018(2):34-36.CHEN L, WANG Z S. Review and protection status of Przewalski's wild horse[J]. Forestry of Xinjiang, 2018(2):34-36. (in Chinese)
[16]	王征. 马鼻肺炎灭活疫苗的研制及其免疫效果的研究[D]. 哈尔滨:东北农业大学, 2012.WANG Z. Preparation and efficacy testing of one inactivated vaccine used for against equine rhinopneumoitis[D]. Harbin:Northeast Agricultural University, 2012. (in Chinese)
[17]	贾洪波. 马传染性鼻肺炎的防治[J]. 吉林畜牧兽医, 2019, 40(11):108-110.JIA H B. Prevention and treatment of equine rhinopneumoitis[J]. Jilin Animal Husbandry and Veterinary Medicine, 2019, 40(11):108-110. (in Chinese)
[18]	陈绍语. 竞技用马的饲养管理[J]. 新疆畜牧业, 2012(7):34-35.CHEN S Y. Feeding management of racing horses[J]. Xinjiang Animal Husbandry, 2012(7):34-35. (in Chinese)
[19]	孙倩, 庞和彪. 新疆焉耆马饲养与管理[J]. 中国畜禽种业, 2017, 13(7):102.SUN Q, PANG H B. Feeding and management of Xinjiang Yanqi Horse[J]. The Chinese Livestock and Poultry Breeding, 2017, 13(7):102. (in Chinese)
[20]	MARENZONI M L, PASSAMONTI F, CAPPELLI K, et al. Clinical, serological and molecular investigations of EHV-1 and EHV-4 in 15 unweaned thoroughbred foals[J]. Vet Rec, 2008, 162(11):337-341.
[21]	ATA E B, ZAGHAWA A, GHAZY A A, et al. Equine herpes virus type-1 infection:etiology, epidemiology, pathogenesis, identification and recent diagnosis[J]. Asian J Epidemiol, 2018, 11(1):34-45.